Functions of corazonin and histamine in light entrainment of the circadian pacemaker in the Madeira cockroach, Rhyparobia maderae
Functions of corazonin and histamine in light entrainment of the circadian pacemaker in the Madeira cockroach, Rhyparobia maderae
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DOI：
10.1002/cne.24133
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发表时间：
2017-04-01
影响因子：
2.5
通讯作者：
Stengl, Monika
中科院分区：
文献类型：
--
作者：
Arendt, Andreas;Baz, El-Sayed;Stengl, Monika
Madeira蟑螂Rhyparobia（Leucophaea）maderae的昼夜节律起搏器位于副髓质（AME）。同侧和对侧组胺能复眼是光夹带所必需的。光脉冲延迟运动活动的节奏在夜间早期和推进它在深夜。因此，不同的神经元通路可能会将光依赖性延迟或提前传递给时钟。注射神经活性物质结合转轮试验表明，GABA，色素分散因子，肌抑制肽（MIPs），和orcokinins（ORCs）的一部分，这两个夹带途径，而allatotropin（AT）只延迟运动节律在深夜。为了进一步表征光夹带，注射组胺和corazonin。组胺注射导致光样相位延迟和提前，表明复眼的神经递质参与了这两个夹带途径。由于注射corazonin仅在主观夜间晚期推进，因此假设corazonin仅是推进途径的一部分。多标记免疫细胞化学结合神经生物素回填表明，一个单一的细胞表达corazonin在视叶，属于一组的中间AME的中间神经元。它共定位GABA和MIP，但不AT或ORC免疫反应性。corazonin免疫反应阳性终末与同侧和对侧复眼的瞳孔光敏感中间神经元投射重叠。因此，我们假设，corazonin-ir内侧神经元整合同侧和对侧光信息的相位推进光夹带通路的一部分，昼夜节律钟。J. Comp.神经元525：1250-1272，2017. (c)2016 Wiley Periodicals，Inc.
The circadian pacemaker of the Madeira cockroach, Rhyparobia (Leucophaea) maderae, is located in the accessory medulla (AME). Ipsi- and contralateral histaminergic compound eyes are required for photic entrainment. Light pulses delay locomotor activity rhythm during the early night and advance it during the late night. Thus, different neuronal pathways might relay either light-dependent delays or advances to the clock. Injections of neuroactive substances combined with running-wheel assays suggested that GABA, pigment-dispersing factor, myoinhibitory peptides (MIPs), and orcokinins (ORCs) were part of both entrainment pathways, whereas allatotropin (AT) only delayed locomotor rhythms at the early night. To characterize photic entrainment further, histamine and corazonin were injected. Histamine injections resulted in light-like phase delays and advances, indicating that the neurotransmitter of the compound eyes participates in both entrainment pathways. Because injections of corazonin only advanced during the late subjective night, it was hypothesized that corazonin is only part of the advance pathway. Multiple-label immunocytochemistry in combination with neurobiotin backfills demonstrated that a single cell expressed corazonin in the optic lobes that belonged to the group of medial AME interneurons. It colocalized GABA and MIP but not AT or ORC immunoreactivity. Corazonin-immunoreactive (-ir) terminals overlapped with projections of putatively light-sensitive interneurons from the ipsi- and contralateral compound eye. Thus, we hypothesize that the corazonin-ir medial neuron integrates ipsi- and contralateral light information as part of the phase-advancing light entrainment pathway to the circadian clock. J. Comp. Neurol. 525:1250-1272, 2017. (c) 2016 Wiley Periodicals, Inc.