Moderation of Arabidopsis Root Sternness by CLAVATA1 and ARABIDOPSIS CRINKLY4 Receptor Kinase Complexes
Moderation of Arabidopsis Root Sternness by CLAVATA1 and ARABIDOPSIS CRINKLY4 Receptor Kinase Complexes
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DOI：
10.1016/j.cub.2013.01.045
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发表时间：
2013-03-04
期刊：
影响因子：
9.2
通讯作者：
Simon, Ruediger
中科院分区：
文献类型：
--
作者：
Stahl, Yvonne;Grabowski, Stephanie;Simon, Ruediger
背景资料：高等植物的根系起源于根分生组织的活动，根分生组织由一组高度特化和持久的干细胞组成。它们的维持和数量由静止中心（QC）细胞和来自分化细胞的反馈信号控制。根分生组织可能已经从结构上不同的芽分生组织演变，但是，没有共同的球员在干控制已被发现到目前为止。结果：我们表明，CLAVATA 1（CLV 1），一个关键的受体激酶在芽干维护，执行类似的，但不同的作用在根分生组织。我们报告说，CLV 1是信号，激活肽配体CLAVATA 3/胚胎周围区域40（CLE 40），与受体激酶ARABIDOPSIS CRINKLY 4（ACR 4）一起限制根干。CLV 1和ACR 4在其远端根分生组织中的表达结构域重叠，并定位于质膜（PM）和胞间连丝（PD），ACR 4优先积累的地方。使用多参数荧光图像光谱（MFIS），我们表明，CLV 1和ACR 4可以形成不同的组成，取决于他们的亚细胞localization.Conclusions：我们假设，这些同源和异源复合物可能差异调节远端根分生组织的维护。我们的结论是，祖先的茎干调控系统的重要组成部分也在根中起作用，CLV 1与ACR 4的特异性相互作用起到缓和和控制根分生组织中的干动态平衡的作用。这两种分生组织类型之间的结构差异可能使ACR 4的这种募集成为必要，用于CLV 1的信号传导。
Background: The root system of higher plants originates from the activity of a root meristem, which comprises a group of highly specialized and long-lasting stem cells. Their maintenance and number is controlled by the quiescent center (QC) cells and by feedback signaling from differentiated cells. Root meristems may have evolved from structurally distinct shoot meristems; however, no common player acting in stemness control has been found so far.Results: We show that CLAVATA1 (CLV1), a key receptor kinase in shoot stemness maintenance, performs a similar but distinct role in root meristems. We report that CLV1 is signaling, activated by the peptide ligand CLAVATA3/EMBRYO SURROUNDING REGION40 (CLE40), together with the receptor kinase ARABIDOPSIS CRINKLY4 (ACR4) to restrict root sternness. Both CLV1 and ACR4 overlap in their expression domains in the distal root meristem and localize to the plasma membrane (PM) and plasmodesmata (PDs), where ACR4 preferentially accumulates. Using multiparameter fluorescence image spectroscopy (MFIS), we show that CLV1 and ACR4 can form homo- and heteromeric complexes that differ in their composition depending on their subcellular localization.Conclusions: We hypothesize that these homo- and heteromeric complexes may differentially regulate distal root meristem maintenance. We conclude that essential components of the ancestral shoot sternness regulatory system also act in the root and that the specific interaction of CLV1 with ACR4 serves to moderate and control sternness homeostasis in the root meristem. The structural differences between these two meristem types may have necessitated this recruitment of ACR4 for signaling by CLV1.