Constitutive and Induced Activities of Defense-Related Enzymes in Aphid-Resistant and Aphid-Susceptible Cultivars of Wheat
Constitutive and Induced Activities of Defense-Related Enzymes in Aphid-Resistant and Aphid-Susceptible Cultivars of Wheat
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DOI：
10.1007/s10886-009-9589-5
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发表时间：
2009-02-01
影响因子：
2.3
通讯作者：
Cai, Qing-Nian
中科院分区：
文献类型：
--
作者：
Han, Ying;Wang, Yu;Cai, Qing-Nian
苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）和过氧化物酶（POD）被认为是寄主植物抗虫性的重要生化标记。以抗、感麦长管蚜小麦品种为材料，分析了这些酶的组成活性。在不同的发育阶段，即，分蘖、茎伸长、剑叶和穗。蚜虫侵染后，在旗叶和穗期测定这些酶的活性。在分蘖期、茎伸长期和剑叶期，抗病品种的PAL活性高于感病品种。蚜虫侵染增强了抗病和感病品种旗叶和穗期的PAL活性水平。抗病品种的PPO活性在各发育阶段均较高。蚜虫侵染诱导一个感病品种的旗叶和穗期PPO活性增加，而抗性品种的诱导较弱。抗病品种在分蘖期、茎伸长期和剑叶期表现出较高的组成型POD活性，而蚜虫侵扰则诱导了所有品种的POD活性，尤其是易感品种。讨论了PAL、PPO和POD在小麦抗蚜中的潜在作用。
Phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) are considered important biochemical markers in host plant resistance against pest insects. Constitutive activity of these enzymes was analyzed in resistant and susceptible wheat cultivars against cereal aphid Sitobion avenae (F.) at various developmental stages, i.e., tillering, stem elongation, flag leaf, and ear. Following aphid infestation, the activity of these enzymes was determined at the flag leaf and ear stages. Resistant cultivars exhibited greater constitutive PAL activity than susceptible ones at the tillering, stem elongation, and flag leaf stages. Aphid infestation enhanced levels of PAL activity in the flag leaf and ear stages in both resistant and susceptible cultivars. Constitutive PPO activity was higher in the resistant cultivars at all developmental stages. Aphid infestation induced increases in PPO activity in the flag leaf and ear stages of one susceptible cultivar, whereas induction in resistant cultivars was weaker. Resistant cultivars showed greater constitutive POD activity in the tillering, stem elongation, and flag leaf stages, while aphid infestation induced POD activity in all cultivars, especially in susceptible ones. The potential role of PAL, PPO, and POD in wheat defense against aphid infestation is discussed.