COMPARATIVE-STUDY OF VIRION STRUCTURE, PROTEIN-COMPOSITION AND GENOMIC DNA OF 3 ASCOVIRUS ISOLATES
COMPARATIVE-STUDY OF VIRION STRUCTURE, PROTEIN-COMPOSITION AND GENOMIC DNA OF 3 ASCOVIRUS ISOLATES
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DOI：
10.1099/0022-1317-71-8-1661
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发表时间：
1990-08-01
影响因子：
3.8
通讯作者：
HAMM, JJ
中科院分区：
文献类型：
--
作者：
FEDERICI, BA;VLAK, JM;HAMM, JJ
比较了从夜蛾夜蛾、草地贪夜蛾和粉纹夜蛾中分离出的三种壳病毒的病毒体的大小和结构、蛋白质组成以及DNA的大小和相关性。来自玉米螺杆菌(HAV)和T.ni(TAV)的分离物的病毒体呈尿囊状(400×130nm)，被包膜并包含内部颗粒，该内部颗粒似乎具有围绕DNA核心的内部脂质双层。草地贪夜蛾分离株(SAV)的病毒体结构和大小相似，但形状为杆状，形成后常闭塞于囊泡状闭塞体中。在每个分离株的纯化病毒体制备物中，检测到至少12种多肽，其大小范围为10K至200K，并且包含约50K的主要种类。 SAV的基因组大小约为140kbp，而TAV和HAV的基因组大小约为180kbp。对 BamHI、HindIII 或 XhoI 产生的三个分离株的 DNA 片段模式分析以及 DNA-DNA 斑点印迹和 Southern 印迹杂交研究表明，HAV 和 TAV 密切相关，但不相同。然而，SAV 的 DNA 并未与其他分离株的 DNA 杂交。因此，来自 T. ni 和 H. zea 的子囊病毒分离株被认为是同一病毒的变种，而来自 S. frugiperda 的子囊病毒分离株是子囊病毒组的单独成员。
The virions of three ascoviruses isolated from the noctuids Heliothis zea, Spodoptera frugiperda and Trichoplusia ni were compared with respect to their size and structure, protein composition and the size and relatedness of their DNAs. The virions of the isolates from H. zea (HAV) and T. ni (TAV) were allantoid in shape (400 .times. 130 nm), enveloped and contained an inner particle which appeared to have an internal lipid bilayer surrounding the DNA core. The virions of the S. frugiperda isolate (SAV) were similar in structure and size, but were bacilliform in shape, and after formation, were often occluded in vesiculate occlusion bodies. In preparations of purified virions of each isolate, at least 12 polypeptides were detected that ranged in size from 10K to 200K and contained a major species of about 50K. The genome of SAV was about 140 kbp in size, whereas those of TAV and HAV were approximately 180 kbp. Analysis of DNA fragment patterns of the three isolates generated with BamHI, HindIII or XhoI, as well as DNA-DNA dot blot and Southern blot hybridization studies, demonstrated that HAV and TAV were closely related but not identical. The DNA from SAV, however, did not hybridize with the DNA from either of the other isolates. Thus the ascovirus isolates from T. ni and H. zea are considered variants of the same virus, whereas the isolate from S. frugiperda is a separate member of the ascovirus group.