Egf1.5 is a second phenoloxidase cascade inhibitor encoded by Microplitis demolitor bracovirus
Egf1.5 is a second phenoloxidase cascade inhibitor encoded by Microplitis demolitor bracovirus
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DOI：
10.1016/j.ibmb.2010.04.009
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发表时间：
2010-07-01
影响因子：
3.8
通讯作者：
Strand, Michael R.
中科院分区：
文献类型：
--
作者：
Lu, Zhiqiang;Beck, Markus H.;Strand, Michael R.
来自多DNA病毒侧沟茧蜂杆状病毒（Microplitis demolitor bracovirus，MdBV）的Egf基因家族的三个成员编码以富含半胱氨酸的基序为特征的新蛋白。先前的研究确定了一个家族成员，Egf 1.0，通过使酚氧化酶活化蛋白酶（PAPS）失活来抑制天蛾血淋巴的黑化。在这里，我们的特点是第二个家庭成员，Egf1.5，它与Egf1.0共享一个相同的富含半胱氨酸的基序，但具有一个扩展的C-末端重复结构域。与Egf1.0相似，Egf1.5抑制M.六。Egf 1.5还结合PAP 1、PAP 3和丝氨酸蛋白酶同源物2（SPH 2）。比较研究表明，Egf 1.5和Egf 1.0类似地抑制了两种鳞翅目昆虫（Pseudoplusia includens和Helicoverpa zea）血浆的黑化，这两种昆虫是M. demolitor和MdBV，以及两种鳞翅目昆虫（M. sexta和mori），它们是非许可宿主。表达研究表明egf1.5和egf1.0在感染MdBV的包涵体毕赤酵母和H.玉米综上所述，我们的结果表明Egf1.5和Egf1.0是功能相似的旁系同源物。(c)2010爱思唯尔有限公司版权所有。
The three-member Egf gene family from the polydnavirus Microplitis demolitor bracovirus (MdBV) encodes novel proteins distinguished by a shared cysteine-rich motif. Prior studies determined that one family member, Egf1.0, inhibits melanization of hemolymph from the moth Manduca sexta by disabling phenoloxidase activating proteinases (PAPS). Here we characterized a second family member, Egf1.5, which shares an identical cysteine-rich motif with Egf1.0, but possesses an extended C-terminal repeat domain. Similar to Egf1.0, Egf1.5 inhibited processing and the amidolytic activity of PAP1 and PAP3 from M. sexta. Egf1.5 also bound PAP1, PAP3 and serine proteinase homolog 2 (SPH2). Comparative studies indicated that Egf1.5 and Egf1.0 similarly inhibited melanization of plasma from two lepidopterans (Pseudoplusia includens and Helicoverpa zea) that are permissive hosts for M. demolitor and MdBV, and two lepidopterans (M. sexta and Bombyx mori) that are nonpermissive hosts. Expression studies showed that transcript abundance of egf1.5 and egf1.0 was also similar in MdBV-infected P. includens and H. zea. Taken together, our results indicate that Egf1.5 and Egf1.0 are functionally similar paralogs. (c) 2010 Elsevier Ltd. All rights reserved.