Cytotoxic effects of plasma-irradiated fullerenol
Cytotoxic effects of plasma-irradiated fullerenol
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DOI：
10.1088/1361-6463/aad510
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发表时间：
2018-08
期刊：
影响因子：
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通讯作者：
Daiki Kanno;Hiromasa Tanaka;K. Ishikawa;H. Hashizume;M. Hori
中科院分区：
文献类型：
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作者：
Daiki Kanno;Hiromasa Tanaka;K. Ishikawa;H. Hashizume;M. Hori
富勒烯醇溶于水，用非平衡大气压等离子体辐照。等离子体辐照通过引入羰基、醚键和插层硝酸根阴离子改变了富勒烯醇溶液。将产生的等离子体辐照的富勒烯醇(PF)加入细胞培养液中。添加PF的培养液对HeLa细胞有明显的细胞毒作用。值得注意的是，在添加PF的细胞培养液中培养的HeLa细胞产生了细胞内的活性氧和氮物种，从而使PF的内吞作用诱导了细胞的凋亡。在添加PF的培养液中发现有诱导细胞凋亡的细胞毒作用。
Fullerenol dissolved in water was irradiated with a non-equilibrium atmospheric pressure plasma. Plasma irradiation altered the fullerenol solution by introducing carbonyl groups, ether bonds, and intercalated nitrate anions. The resulting plasma-irradiated fullerenol (PF) was added in cell culture medium. PF-supplemented medium exhibited a cytotoxic effect on HeLa cells. Notably, HeLa cells cultured in the PF-added cell culture medium generated intracellular reactive oxygen and nitrogen species, such that the endocytosis of PF induced the apoptotic cell death. The cytotoxic effect inducing apoptosis was found in the PF-supplemented medium.