INITIAL MICROBIAL ADHESION IS A DETERMINANT FOR THE STRENGTH OF BIOFILM ADHESION
INITIAL MICROBIAL ADHESION IS A DETERMINANT FOR THE STRENGTH OF BIOFILM ADHESION
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DOI：
10.1016/0378-1097(95)00103-c
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发表时间：
1995-05-15
影响因子：
2.1
通讯作者：
VANDERMEI, HC
中科院分区：
文献类型：
--
作者：
BUSSCHER, HJ;BOS, R;VANDERMEI, HC
本文提出了一个关于微生物初始粘附在整个生物膜形成过程中的重要性的假设。该假设是基于动态剪切条件存在于许多环境中的认识，例如在口腔中，或在岩石和船体上。认识到一旦最初粘附的生物体和表面（通常通过所谓的“调节膜”构成）之间的结合被打破，整个生物膜在高剪切过程中分离，很明显，研究应该关注分离而不是粘附。实验是在一个平行的板腔中进行的，其中试图通过施加由气泡通过引起的高剪切力，使空气-液体界面从玻璃上分离粘附的口腔链球菌。从裸露的玻璃和最初粘附的放线菌菌株中分离链球菌似乎没有发生。然而，当黏附通过唾液调节膜介导时，黏附链球菌发生了实质性的脱离，可能是因为调节膜的黏附失败。
This paper presents a hypothesis on the importance of initial microbial adhesion in the overall process of biofilm formation. The hypothesis is based on the realization that dynamic shear conditions exist in many environments, such as in the oral cavity, or on rocks and ship hulls. Recognizing that an entire biofilm ig detached during high shear once the bond between the initially adhering organisms and a surface (often constituted through a so-called 'conditioning film') is broken, it becomes clear that research should focus on detachment rather than adhesion. Experiments were done in a parallel plate now chamber in which attempts were made to detach adhering oral streptococci from glass by applying a high shear caused by the passage of a bubble, giving an air-liquid interface. Detachment of streptococci from bare glass and from an initially adhering actinomycete strain appeared not to occur. However, substantial detachment of adhering streptococci occurred when adhesion was mediated through a salivary conditioning film, presumably because of cohesive failure in the conditioning film.