Carbapenem inactivation method using bacterial lysate and MOPS (LCIM): a very sensitive method for detecting carbapenemase-producing Acinetobacter species.

Carbapenem inactivation method using bacterial lysate and MOPS (LCIM): a very sensitive method for detecting carbapenemase-producing Acinetobacter species.
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使用细菌裂解物和 MOPS 的碳青霉烯灭活方法 (LCIM)：一种非常灵敏的检测产生碳青霉烯酶的不动杆菌属的方法。

DOI：
10.1093/jac/dkaa238
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发表时间：
2020
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
通讯作者：
K. Tateda
K. Tateda
中科院分区：
--
文献类型：
--
作者：
Kageto Yamada;K. Aoki;Tatsuya Nagasawa;W. Imai;Masakazu Sasaki;H. Murakami;Toshisuke Morita;Y. Ishii;K. Tateda

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目标 产碳青霉烯水解D类β-内酰胺酶（CHDL）不动杆菌属的检测对了解抗生素耐药性至关重要。在这项研究中，我们比较了碳青霉烯灭活法（CIM）的现有检测技术，使用产CHDL的不动杆菌属，并开发了一种使用细菌裂解物（裂解物CIM; LCIM）的改良方法。 方法 共检出159株不动杆菌属。(102产碳青霉烯酶菌和57个非产碳青霉烯酶菌）和14个假单胞菌属。检测了7例碳青霉烯酶生产者和7例非生产者。用这些菌株比较了改良CIM、简化CIM、CIMTris、Triton-CIM和LCIM。与CIM不同，LCIM包括在20 mM MOPS缓冲液而不是水中使用2.0% Triton X-100（v/v）的更长孵育期（4 h）。 结果 改良CIM、简化CIM、CIMTris、Triton-CIM和LCIM的敏感性/特异性分别为71.6%/100%、66.1%/89.1%、88.1%/95.3%、80.7%/100%和97.2%/100%。LCIM最敏感、特异。 结论 使用细菌裂解物和MOPS增加了CIM检测产CHDL不动杆菌属的灵敏度。
OBJECTIVES Detection of carbapenem-hydrolysing class D β-lactamase (CHDL)-producing Acinetobacter spp. is critical for understanding antibiotic resistance. In this study, we compared the available detection techniques derived from the carbapenem inactivation method (CIM), using CHDL-producing Acinetobacter spp., and developed a modified method that uses bacterial lysate (lysate CIM; LCIM). METHODS A total of 159 Acinetobacter spp. (102 carbapenemase producers and 57 non-producers) and 14 Pseudomonas spp. (7 carbapenemase producers and 7 non-producers) were tested. Modified CIM, simplified CIM, CIMTris, Triton-CIM and LCIM were compared using these strains. Distinct from the CIM, LCIM includes a longer incubation period (4 h) with 2.0% Triton X-100 (v/v) in 20 mM MOPS buffer instead of water. RESULTS The sensitivity/specificity of the modified CIM, simplified CIM, CIMTris, Triton-CIM and LCIM were 71.6%/100%, 66.1%/89.1%, 88.1%/95.3%, 80.7%/100% and 97.2%/100%, respectively. LCIM was the most sensitive and specific. CONCLUSIONS Use of bacterial lysate and MOPS increased the sensitivity of the CIM in detecting CHDL-producing Acinetobacter spp.