Paeoniflorin reduces the inflammatory response of THP-1 cells by up-regulating microRNA-124 : Paeoniflorin reduces the inflammatory response of THP-1 cells through microRNA-124.
Paeoniflorin reduces the inflammatory response of THP-1 cells by up-regulating microRNA-124 : Paeoniflorin reduces the inflammatory response of THP-1 cells through microRNA-124.
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芍药苷通过上调microRNA-124降低THP-1细胞的炎症反应：芍药苷通过microRNA-124降低THP-1细胞的炎症反应。
DOI：
10.1007/s13258-021-01083-2
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发表时间：
2021-06
期刊：
影响因子：
2.1
通讯作者：
Yu T
中科院分区：
文献类型：
--
作者：
Huang D;Li Z;Chen Y;Fan Y;Yu T
巨噬细胞活化和炎性细胞因子的释放是系统性红斑狼疮(SLE)进展的主要原因。MicroRNA(MiRNA)-124参与巨噬细胞的调节，是炎症和免疫的关键调节因子。探讨芍药苷(PF)是否通过miR-124调节巨噬细胞的生物学功能。采用RT-PCR、WB、ELISA法、CCK-8法和流式细胞术检测PF通过miR-124对THP-1细胞生物学功能的调节。PF明显抑制THP-1细胞增殖，促进细胞凋亡，抑制IL-6、肿瘤坏死因子-α和IL-1β的释放。RT-PCR结果显示，PF可上调THP-1细胞miR-124的表达。功能恢复实验表明，与脂多糖 + Mimic-NC组相比， + Mimic-124Mimic能显著抑制THP-1细胞的增殖和IL-6、α、IL-1β的释放，促进细胞的凋亡。此外，与脂多糖 + PF + 抑制剂-NC组相比， + PF + -12 4抑制剂能显著促进THP-1细胞的增殖和IL-6、α、IL-1β的释放，但抑制细胞的凋亡。PF通过下调miR-124抑制THP-1细胞的增殖和炎症反应，促进THP-1细胞的凋亡。
The activation of macrophages and the release of inflammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of inflammation and immunity. To explore whether paeoniflorin (PF) regulates the biological functions of macrophages depends on miR-124. RT-PCR, WB, ELISA, CCK-8 and flow cytometry were used to evaluate that PF regulated the biological functions of THP-1 cells through miR-124. PF significantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1βin THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS + mimic-NC group, LPS + miR-124 mimic significantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS + PF + inhibitor-NC group, LPS + PF + miR-124 inhibitor significantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells. By down-regulating miR-124, PF inhibits the proliferation and inflammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.
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