Changes in enzymes activity, substrate utilization pattern and diversity of soil microbial communities under cadmium pollution.
Changes in enzymes activity, substrate utilization pattern and diversity of soil microbial communities under cadmium pollution.
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DOI：
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发表时间：
2005
影响因子：
6.9
通讯作者：
Akmal Muhammad;Hai-zhen Wang;Jian-jun Wu;Jian-ming Xu;De-fu Xu
中科院分区：
文献类型：
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作者：
Akmal Muhammad;Hai-zhen Wang;Jian-jun Wu;Jian-ming Xu;De-fu Xu
近年来，重金属污染越来越受到人们的关注，主要是因为公众对环境问题的认识。在25 ℃培养60 d的条件下，研究了添加0、20、40、60、80和100 mg/kg Cd对土壤酶活性、底物利用模式和微生物群落多样性的影响。重金属处理后0、15、30、45和60 d土壤酶活性均显著下降，至60 d，100 mg/kg Cd处理土壤脲酶、酸性磷酸酶和脱氢酶活性分别比对照降低50.1%、47.4%和39.8%。在60 DAA时，通过对土壤微生物群落基质利用模式的研究表明，Cd处理显著抑制了土壤微生物群落的功能活性，对单一碳源利用的多变量分析表明，80和100 mg/kg Cd处理的土壤微生物群落利用模式存在显著差异。结果表明，随着Cd浓度的增加，土壤DGGE图谱逐渐简化，不同Cd处理的DGGE图谱聚类结果与对照差异均在50%以上。
Heavy metal pollution has received increasing attention in recent years mainly because of the public awareness of environmental issues. In this study we have evaluated the effect of cadmium (Cd) on enzymes activity, substrate utilization pattern and diversity of microbial communities in soil spiked with 0, 20, 40, 60, 80, and 100 mg/kg Cd, during 60 d of incubation at 25 degrees C. Enzyme activities determined at 0, 15, 30, 45, and 60 d after heavy metal application (DAA) showed marked declines for various Cd treatments, and up to 60 DAA, 100 mg/kg Cd resulted in 50.1%, 47.4%, and 39.8% decreases in soil urease, acid phosphatase and dehydrogenase activities, respectively to control. At 60 DAA, substrate utilization pattern of soil microbial communities determined by inoculating Biolog ECO plates indicated that Cd addition had markedly inhibited the functional activity of soil microbial communities and multivariate analysis of sole carbon source utilization showed significantly different utilization patterns for 80 and 100 mg/kg Cd treatments. The structural diversity of soil microbial communities assessed by PCR-DGGE method at 60 DAA, illustrated that DGGE patterns in soil simplified with increasing Cd concentration, and clustering of DGGE profiles for various Cd treatments revealed that they had more than 50% difference with that of control.