Behavioral changes in rats after acute, chronic and repeated administration of interleukin-1 beta: relevance for affective disorders
Behavioral changes in rats after acute, chronic and repeated administration of interleukin-1 beta: relevance for affective disorders
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DOI：
10.1016/s0165-0327(02)00118-0
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发表时间：
2003-11-01
影响因子：
6.6
通讯作者：
Maes, M
中科院分区：
文献类型：
--
作者：
Bonaccorso, S;Maier, SF;Maes, M
背景：重组大鼠白细胞介素 1β (IL-1β) 的急性、外周和中枢给药已被证明可减少大鼠的社交探索和运动活动，并诱导大鼠脑生物胺的改变。本研究的目的是检查大鼠急性、重复和长期注射 IL-1β 是否会干扰穿梭盒逃脱学习（一种焦虑和抑郁样行为模型）。方法：实验中使用 64 只成年雄性无病毒 Sprague-Dawley 大鼠（体重 200-300 g），四只一组，在 25 ℃ 下饲养，光：暗周期为 12:12。他们被分为8组，即4组对照组和4组实验组。后者被分为急性组，接受单次腹膜内 (I.P.) IL-1beta 攻击（1 小时和 24 小时后在穿梭箱中进行测试）；慢性组每天进行腹腔注射。注射IL-1beta 7天（1小时后在穿梭箱中测试）；以及重复给药组，即一次IP。第一天注射，第七天第二次挑战（1小时后在穿梭箱中测试）。对照动物遵循相同的注射和测试时间表，但接受腹腔注射治疗。用生理盐水。结果：接受一次IL-1β攻击并在1小时（P=0.001）和24小时（P=0.002）后进行测试的急性组在逃脱试验中显示出显着的时间延长。连续7天长期接受IL-1β治疗的动物在逃脱试验中表现出潜伏期显着增加（P=0.0001）。第一天重复给予IL-1β并在第七天重复给予第二次IL-1β并没有显着改变逃脱试验中的时间延长。讨论：急性和慢性施用 IL-1β 显着增加逃避足部电击的潜伏期，而重复施用 IL-1β 不会诱导这些行为反应的敏化。 (C) 2002 年，Elsevier B.V. 出版
Background: Acute, peripheral and central administration of recombinant rat interleukin-1beta (IL-1beta) has been shown to decrease social exploration and locomotor activity and to induce alterations in brain biogenic amines in rats. The aims of this study were to examine whether acute, repeated and chronic administration of IL-1beta to rats may interfere with shuttle box escape learning, a model for anxiety- and depression-like behavior. Methods: Sixty-four adult male viral-free Sprague-Dawley rats (200-300 g weight) housed in groups of four at 25 degreesC with a 12:12 light:dark cycle were used in the experiments. They were divided into 8 groups, i.e. 4 control and 4 experimental. The latter were divided into an acute group receiving a single intra-peritoneally (I.P.) challenge of IL-1beta (tested at the shuttle box 1 and 24 h later); a chronic group with daily I.P. injections of IL-1beta for 7 days (tested at the shuttle box 1 h later); and a group with repeated administration, i.e. one I.P. injection on the first day and a second challenge on the seventh day (tested at the shuttle box 1 h later). The control animals followed the same injecting and testing schedule but were treated I.P. with saline. Results: The acute group treated with one IL-1beta challenge and tested 1 (P=0.001) and 24 h (P=0.002) later showed significant time elongations in the escape trials. The animals treated chronically with IL-1beta for seven consecutive days showed a significant increase in the latency at the escape trials (P=0.0001). Repeated administration of IL-1beta on the first day and a second on the seventh day did not significantly alter the time elongation in the escape trial. Discussion: Acute and chronic administration of IL-1beta significantly increase the latency of escape to a foot shock, whereas repeated IL-1beta administration does not induce a sensitization of these behavioral responses. (C) 2002 Published by Elsevier B.V.