Histopathology of periodontal disease in gnotobiotic rats monoinfected with Eikenella corrodens.
Histopathology of periodontal disease in gnotobiotic rats monoinfected with Eikenella corrodens.
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单一感染 Eikenella corrodens 的无菌大鼠牙周病的组织病理学。
DOI：
10.1111/j.1600-0765.1978.tb00162.x
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发表时间：
1978
影响因子：
3.5
通讯作者：
S. Socransky
中科院分区：
文献类型：
--
作者：
M. Listgarten;D. Johnson;A. Nowotny;A. Tanner;S. Socransky
用从人类牙周炎病变中分离的革兰氏阴性杆菌Eikenella corrodens单感染的非细菌性大鼠，研究牙周病的组织病理学。感染后2-14周，动物成对处死，通过目视检查对磨牙节段的牙周病严重程度进行评分。病变最早出现在上颌磨牙，且上颌感染后较下颌感染后更早。感染后2周采集的标本显示，在交界上皮的冠状部分有毛发嵌塞，一些白细胞移出，但结缔组织中没有细胞浸润或血管异常的证据。3-5周时，在结缔组织内形成一个火山口样凹陷，其表面覆盖着被撞击碎片破坏的连续上皮衬里。在牙骨质-釉质交界处的顶端，上皮附着物几乎没有移动。6-8周之间。牙间上皮衬里溃疡。在结缔组织内通常观察到嵌塞的毛发和其他碎屑，但结缔组织中不含浆细胞，仅含有分离的淋巴细胞。骨破坏的特征是明显的骨破坏活性。牙齿表面保持明显无粘附细菌。事实上，仅在一个13周样本中观察到与撞击碎片相关的细菌。与人类和其他一些物种的牙周破坏的进展相反，这些动物中疾病过程的最顶端范围位于沿着牙间组织的中心部分，而不是沿着牙根表面。在这些动物中，原发性甲状腺炎的严重程度与它们对感染生物体的细胞介导的免疫反应之间似乎不存在相关性或可能存在负相关性。
The histopathology of periodontal disease was studies in gnotobiotic rats monoinfected with Eikenella corrodens, a Gram-negative rod isolated from a human periodontitis lesion. Animals were sacrificed in pairs from 2–14 weeks after infection and the molar segments scored for severity of periodontal disease by visual inspection. the lesions first appeared in the maxillary molars and were after infection in the maxilla than in the mandible. Specimens taken 2 weeks after infection showed hair impaction in the coronal part of the junctional epithelium and some leukocyte emigration, but no evidence of a cellular infiltrate or vascular anomalies in the connective tissue. Between 3–5 weeks, a crater-like depression developed interdentally within the connective tissue, the surface of which was Covered by a continous epitheliel lining disrupted by impacted debris. Little mogrationof the epithelial attachment was noted apical to the cemento-enamel junction. Between 6–8 weeks. The interdental epithelial lining became ulcerated. Impacted hair and other debris were commonly noted within the connective tissue which, however, remained free of plasma cells and only contained isolated lymphocytes. Bone destruction was characterized by marked osteoclastic activity. The tooth Surfaces remained remarkably free of adherent bacteria. In fact, bacteria were only observed in one 13-week specimen, in association with impacted debris. Contrary to the progress of periodontal breakdown in man and some other species, the most apical extent of the disease process in these animals was located along the central portion of the interdental tissues, rather than along the root surfaces. No correlation or possibly an inverse correlation seemed to exist between the severity of priodontal disease in these animals and their cell-madiated immune response to the infecting organism.