Distinct effects of two CD44 isoforms on tumor growth in vivo.
Distinct effects of two CD44 isoforms on tumor growth in vivo.
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DOI：
10.1084/jem.174.4.859
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发表时间：
1991-10-01
期刊：
影响因子：
--
通讯作者：
Stamenkovic I
中科院分区：
文献类型：
--
作者：
Sy MS;Guo YJ;Stamenkovic I
肿瘤的生长在一定程度上取决于肿瘤细胞和宿主组织的细胞外基质之间的相互作用。介导细胞-基质相互作用的细胞表面糖蛋白CD44/PGP-1在许多类型的恶性肿瘤中表达增加，但CD44在肿瘤生长中的作用在很大程度上尚不清楚。最近，CD44的两种亚型被鉴定出来：一种是80-90kD的形式，它与细胞结合的透明质酸有很高的亲和力；另一种是150kD的形式，它不介导与透明质酸表面的附着。在这项工作中，人B细胞淋巴瘤细胞稳定地转染了编码这两种CD44亚型中的任何一种的cDNA克隆，并比较了其在裸鼠体内的致瘤性和转移潜能。表达80-90kD形式的CD44，而不表达150kD形式的CD44，大大增强了淋巴瘤细胞的局部肿瘤形成和转移倾向。我们的结果提示，CD44多肽可能在体内调节原发和转移肿瘤的发展中发挥重要作用。
Tumor growth is dependent in part on interactions between tumor cells and the extracellular matrix of host tissues. Expression of the cell surface glycoprotein CD44/Pgp-1, which mediates cell-substrate interactions is increased in many types of malignancies, but the role of CD44 in tumor growth is largely undefined. Recently, two isoforms of CD44 have been identified: an 80-90 kD form, which has high affinity for cell bound hyaluronate and a 150 kD form which does not mediate attachment to hyaluronate-coated surfaces. In this work, human B cell lymphoma cells stably transfected with cDNA clones encoding either of the two CD44 isoforms were compared for tumorigenicity and metastatic potential in nude mice. Expression of the 80-90 kD form but not the 150 kD form of CD44 greatly enhanced both local tumor formation and metastatic proclivity of the lymphoma cells. Our results suggest that CD44 polypeptides may play an important role in regulating primary and metastatic tumor development in vivo.