Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen
Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen
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DOI：
10.1016/j.bbrc.2015.06.159
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发表时间：
2015-08-21
影响因子：
3.1
通讯作者：
Park, Choon-Keun
中科院分区：
文献类型：
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作者：
Lee, Sang-Hee;Park, Choon-Keun
磁化水被定义为已经通过磁体并显示出增加的细胞渗透性和供电子特性的水。这些属性可以保护膜免受损伤，并清除哺乳动物细胞中的活性氧（ROS）。研究了以牛血清白蛋白（BSA）为抗氧化剂的改良磁处理精液稀释液对猪精子凋亡的影响。将精液稀释在磁化稀释液（OG和6000G）中（含或不含BSA、COG + BSA和6000G + BSA），并使用流式细胞术基于活精子的活力、顶体反应和H2O2水平分析精子。精子在18摄氏度下保存11天。6000G + BSA组的存活率显著高于其他处理组（P < 0.05）。6000G + BSA组顶体反应明显低于其他处理组（P < 0.05）。6000G + BSA组活精子细胞内H2O2水平显著低于其他处理组（P < 0.05）。结果表明，磁化稀释液对猪精子液态保存具有一定的抗氧化作用。(C)2015 Elsevier Inc. All rights reserved.
Magnetized water is defined as water that has passed through a magnet and shows increased permeability into cells and electron-donating characteristics. These attributes can protect against membrane damage and remove reactive oxygen species (ROS) in mammalian cells. We explored the effects of improved magnetized semen extenders containing bovine serum albumin (BSA) as antioxidants on apoptosis in boar sperm. Ejaculated semen was diluted in magnetized extender (OG and 6000G) with or without BSA COG + BSA and 6000G + BSA), and sperm were analyzed based on viability, acrosome reaction, and H2O2 level of live sperm using flow cytometry. Sperm were then preserved for 11 days at 18 degrees C. We found that viability was significantly higher in 6000G + BSA than under the other treatments (P < 0.05). The acrosome reaction was significantly lower in the 6000G + BSA group compared with the other treatments (P < 0.05). Live sperm with high intracellular H2O2 level were significantly lower in the 6000G + BSA group than under other treatments (P < 0.05). Based on our results, magnetized extenders have antioxidative effects on the liquid preservation of boar sperm. (C) 2015 Elsevier Inc. All rights reserved.