Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
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DOI：
10.1021/bc2000066
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发表时间：
2011-04-01
影响因子：
4.7
通讯作者：
Yun, Hyungdon
中科院分区：
文献类型：
--
作者：
Ayyadurai, Niraikulam;Prabhu, Nadarajan Saravanan;Yun, Hyungdon
我们描述了结合高碘酸盐化学和非天然氨基酸掺入的简单生物共轭策略。 3,4-二羟基-L-苯丙氨酸的残基特异性掺入可以改变蛋白质缀合到聚合物中的特性。均质修饰的蛋白质将产生与氨基多糖的亲核基团共价缀合的醌残基。这种新颖的方法对于广泛用于制备蛋白质缀合物和合成生物学应用具有广阔的前景。
We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation of 3,4-dihydroxy-L-phenylalanine can alter the properties of protein to conjugate into the polymers. The homogeneously modified protein will yield quinone residues that are covalently conjugated to nucleophilic groups of the amino polysaccharide. This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications.