Assessment of chemical carcinogen-induced transforming activity using BALB/c-3T3 cells
Assessment of chemical carcinogen-induced transforming activity using BALB/c-3T3 cells
复制标题
使用 BALB/c-3T3 细胞评估化学致癌物诱导的转化活性
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
E. Matthews
中科院分区：
文献类型：
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作者：
E. Matthews
BALB/c-3T3细胞转化试验以培养的哺乳动物细胞为靶点，评估了试验化学物质的形态转化潜力。该检测是半定量的，采用了来自原始BALB/c-3T3小鼠细胞系的接触抑制克隆。转化活性，或焦点，被认为是一层致密的形态改变的细胞叠加在正常细胞表型的单层上。据报道，在标准化验中，转化活性的诱导与啮齿动物生物化验中许多测试化学物质的致癌性有很好的相关性；然而，标准化验并不能检测所有化学类别的致癌物。本报告描述了标准BALB/c-3T3细胞转化试验的操作规程。此外，它还提供了测试验收标准，并讨论了评估转化活性数据的方法。
The BALB/c-3T3 cell transformation assay evaluates the morphologic transforming potential of test chemicals using cultured mammalian cells as targets. The assay is semiquantitative and employs a contact-inhibited clone of cells derived from the original BALB/c-3T3 mouse cell line. Transforming activity, or a focus, is recognized as a dense layer of morphologically altered cells superimposed on a monolayer of the normal cell phenotype. The induction of transforming activity in a standard assay has been reported to correlate well with the carcinogenicity of many test chemicals in rodent bioassay; however, the standard assay does not detect carcinogens of all chemical classes. This report describes an operational protocol for the standard BALB/c-3T3 cell transformation assay. In addition, it provides assay acceptance criteria and a discussion of a method to evaluate transforming activity data.