Genetic diversity of clinical and environmental strains of Salmollella enterica serotype Weltevreden isolated in Malaysia
Genetic diversity of clinical and environmental strains of Salmollella enterica serotype Weltevreden isolated in Malaysia
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DOI：
10.1128/jcm.40.7.2498-2503.2002
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发表时间：
2002-07-01
影响因子：
9.4
通讯作者：
Puthucheary, SD
中科院分区：
文献类型：
--
作者：
Thong, KL;Goh, YL;Puthucheary, SD
据报道，马来西亚由肠沙门氏菌血清型 Weltevreden 引起的食源性沙门氏菌病的发病率正在增加。脉冲场凝胶电泳 (PFGE) 亚型分型方法用于评估人类和环境中沙门氏菌血清型 Weltevreden 菌株的遗传多样性和克隆性程度。对来自 95 种 Weltevreden 血清型沙门氏菌菌株的 XbaI 消化的染色体 DNA 进行 PFGE，得到 39 个不同的谱，具有广泛的 Dice 系数（0.27 至 1.00），表明 PFGE 具有很强的辨别力，并且临床和环境分离株中存在沙门氏菌血清型 Weltevreden 的多个克隆。一种主要脉冲型（脉冲型 X1/X2）的菌株似乎是该地区的地方病，因为它们在 1996 年至 2001 年间不断从沙门氏菌病患者和生蔬菜中发现。此外，来自人类、蔬菜和牛肉的分离株之间共享相似的 PFGE 谱，间接证明沙诺氏菌病可能从受污染的生蔬菜和肉类传播给人类。此外，在来自一个生鲜市场的蔬菜样本中发现的分离株中重复出现 PFGE 图谱 X21，表明该克隆的持续存在。生鲜市场的环境可能是蔬菜与 Weltevreden 血清型沙门氏菌交叉污染的主要来源。抗生素敏感性测试表明，Weltevreden 血清型沙门氏菌临床分离株仍对药物敏感，但蔬菜分离株对至少两种抗生素具有耐药性。据我们所知，这是第一项比较马来西亚 Weltevreden 血清型沙门氏菌临床和环境分离株的研究。
The incidence of food-borne salmonellosis due to Salmonella enterica serotype Weltevreden is reported to be on the increase in Malaysia. The pulsed-field gel electrophoresis (PFGE) subtyping method was used to assess the extent of genetic diversity and clonality of Salmonella serotype Weltevreden strains from humans and the environment. PFGE of XbaI-digested chromosomal DNA from 95 strains of Salmonella serotype Weltevreden gave 39 distinct profiles with a wide range of Dice coefficients (0.27 to 1.00), indicating that PFGE is very discriminative and that multiple clones of Salmonella serotype Weltevreden exist among clinical and environmental isolates. Strains of one dominant pulsotype (pulsotype X1/X2) appeared to be endemic in this region, as they were consistently recovered from humans with salmonellosis between 1996 and 2001 and from raw vegetables. In addition, the sharing of similar PFGE profiles among isolates from humans, vegetables, and beef provides indirect evidence of the possible transmission of sahnonellosis from contaminated raw vegetables and meat to humans. Furthermore, the recurrence of PFGE profile X21 among isolates found in samples of vegetables from one wet market indicated the persistence of this clone. The environment in the wet markets may represent a major source of cross-contamination of vegetables with Salmonella serotype Weltevreden. Antibiotic sensitivity tests showed that the clinical isolates of Salmonella serotype Weltevreden remained drug sensitive but that the vegetable isolates were resistant to at least two antibiotics. To the best of our knowledge, this is the first study to compare clinical and environmental isolates of Salmonella serotype Weltevreden in Malaysia.