Therapeutic vaccination with vhs(-) herpes simplex virus reduces the severity of recurrent herpetic stromal keratitis in mice.
Therapeutic vaccination with vhs(-) herpes simplex virus reduces the severity of recurrent herpetic stromal keratitis in mice.
复制标题
治疗性疫苗接种 vhs(-) 单纯疱疹病毒可降低小鼠复发性疱疹性基质角膜炎的严重程度。
DOI：
10.1099/0022-1317-83-10-2361
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Stuart,PatrickM
中科院分区：
文献类型：
--
作者：
Keadle,TammieL;Laycock,KeithA;Morris,JessicaL;Leib,DavidA;Morrison,LyndaA;Pepose,JayS;Stuart,PatrickM
Virion host shutoff (vhs)-deficient herpes simplex virus (HSV) was tested as a therapeutic vaccine in a mouse model of UV light-induced recurrent herpetic stromal keratitis. Four weeks after primary corneal infection, mice were vaccinated intraperitoneally with vhs−vaccine or control. Four weeks after vaccination, the eyes of latently infected mice were UV-B irradiated to induce recurrent virus shedding and disease. Post-irradiation corneal opacity in latently infected, vhs−-vaccinated mice was significantly reduced compared to control-vaccinated mice (P=0·007 to 0·035). The incidence and duration of recurrent virus shedding were the same in both groups. Antibody titres were increased (P=0·05) and delayed type hypersensitive responses were unaffected by vhs−vaccination. Combined with studies using different vaccination timing and vhs−genotypes, these data suggest that deletion of vhs is a useful strategy in the development of a therapeutic HSV vaccine, and that temporal and genetic factors influence vaccination outcome.
