In Vitro Biocompatibility Evaluation of a Root Canal Filling Material That Expands on Water Sorption
In Vitro Biocompatibility Evaluation of a Root Canal Filling Material That Expands on Water Sorption
复制标题
DOI：
10.1016/j.joen.2013.03.003
复制
发表时间：
2013-07-01
影响因子：
4.2
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Eid, Ashraf A.;Nikonov, Sergey Y.;Tay, Franklin R.
简介：CPoint 是一种聚合物牙髓尖，利用水引起的非各向同性径向膨胀来适应根管不规则性。本研究评估了 CPoint 对成牙本质细胞样细胞的活力和矿化潜力的影响。方法：使用大鼠成牙本质细胞样细胞系 (MDPC-23) 评估 CPoint 和市售古塔胶点的生物相容性。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定、流式细胞术和共焦激光扫描显微镜评估细胞活力。在存在根充填材料的情况下，通过检查成骨基因标记物表达（定量实时聚合酶链反应）、碱性磷酸酶活性、茜素红 S 测定和透射电子显微镜的变化来评估 MDPC-23 细胞的矿化潜力。结果：与古塔胶和聚四氟乙烯相比，CPoint 显示出更高的初始细胞毒性 (P < .05)，在 4 个浸泡周期后变得不显着。 CPoint 和古塔胶的洗脱液在 1:1 浓度下也存在显着差异 (P < .05)，但在 1:10 或 1:100 浓度下则没有显着差异。流式细胞术和共焦激光扫描显微镜证明，两种材料均诱导细胞凋亡引起的质膜通透性变化最小。与Teflon阴性对照相比，CPoint和古塔胶组显示大多数成骨基因标记物上调，但牙本质唾液酸磷蛋白下调。 CPoint 和古塔胶的碱性磷酸酶活性和茜素红测定均显着高于 Teflon，但彼此之间没有显着差异 (P > .05)。透射电子显微镜显示所有 3 组都有离散的结节状电子致密矿化灶。结论：CPoint 的体外生物相容性与古塔胶相当，在洗脱潜在毒性成分后对成骨的不利影响最小。
Introduction: CPoint is a polymeric endodontic point that takes advantage of water-induced, non-isotropic radial expansion to adapt to canal irregularities. This study evaluated the effects of CPoint on the viability and mineralization potential of odontoblast-like cells. Methods: The biocompatibility of CPoint and commercially available gutta-percha points was evaluated by using a rat odontoblast-like cell line (MDPC-23). Cell viability was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and confocal laser scanning microscopy. The mineralization potential of MDPC-23 cells, in the presence of the root-filling materials, was evaluated by examining the changes in osteogenic gene marker expression (quantitative real-time polymerase chain reaction), alkaline phosphatase activity, alizarin red S assay, and transmission electron microscopy. Results: CPoint showed higher initial cytotoxicity compared with gutta-percha and Teflon (P < .05), which became nonsignificant after 4 immersion cycles. Significant differences were also found between eluents from CPoint and gutta-percha at 1:1 concentration (P < .05) but not at 1:10 or 1:100 concentration. Both materials induced minimal apoptosis-induced alteration in plasma membrane permeability, as evidenced by flow cytometry and confocal laser scanning microscopy. Compared with the Teflon negative control, CPoint and gutta-percha groups showed upregulation of most osteogenic gene markers except for dentin sialophosphoprotein, which was down-regulated. Alkaline phosphatase activity and alizarin red assay for CPoint and gutta-percha were both significantly higher than for Teflon but not significantly different from each other (P > .05). Transmission electron microscopy showed discrete nodular electron-dense mineralization foci in all 3 groups. Conclusions: The in vitro biocompatibility of CPoint is comparable to gutta-percha with minimal adverse effects on osteogenesis after elution of potentially toxic components.