Effects of stretching on the basement membrane structure in the soleus muscle of Wistar rats
Effects of stretching on the basement membrane structure in the soleus muscle of Wistar rats
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DOI：
10.1007/s00795-022-00335-8
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发表时间：
2022-09-15
影响因子：
1.8
通讯作者：
Shigeyoshi, Yasufumi
中科院分区：
文献类型：
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作者：
Kanazawa, Yuji;Takahashi, Tatsuo;Shigeyoshi, Yasufumi
基底膜（BM）主要由IV型胶原蛋白组成，在肌肉纤维的维持、保护和恢复中发挥着重要作用。IV型胶原蛋白的表达是通过合成和降解因子之间的平衡来维持的，其变化取决于肌肉活动的水平。例如，运动增加IV型胶原蛋白的合成，而不运动则减少IV型胶原蛋白的合成。然而，拉伸对BM结构的影响仍不清楚。因此，为了研究拉伸对骨骼肌BM的影响，我们连续拉伸大鼠比目鱼肌，并使用定量聚合酶链反应（qPCR），蛋白质印迹，酶谱，免疫组织化学和电子显微镜检查BM相关因子和结构的表达变化。结果表明，拉伸增加了基质金属蛋白酶14（MMP 14）的表达和MMP 2的活性，降低了IV型胶原的表达和致密板的宽度在比目鱼肌。这些结果表明，拉伸促进大鼠比目鱼肌BM降解。本研究的结果表明，拉伸对骨骼肌的新的影响，并可能有助于拉伸在康复和运动领域的新用途。
The basement membrane (BM), mainly composed of collagen IV, plays an important role in the maintenance, protection, and recovery of muscle fibers. Collagen IV expression is maintained by the balance between synthetic and degradative factors, which changes depending on the level of muscle activity. For example, exercise increases collagen IV synthesis, whereas inactivity decreases collagen IV synthesis. However, the effects of stretching on the BM structure remain unclear. Therefore, to investigate the effects of stretching on the BM of the skeletal muscle, we continuously applied stretching to the rat soleus muscle and examined the altered expression of BM-related factors and structure using quantitative polymerase chain reaction (qPCR), western blotting, zymography, immunohistochemistry, and electron microscopy. The results show that stretching increased the matrix metalloproteinase 14 (MMP14) expression and MMP2 activity, and decreased the collagen IV expression and width of the lamina densa in the soleus muscle. These results suggest that stretching promotes BM degradation in the rat soleus muscle. The findings of this study indicate a new influence of stretching on skeletal muscles, and may contribute to the new use of stretching in rehabilitation and sports fields.