Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry
Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry
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DOI：
10.1016/s0006-3495(00)76297-7
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发表时间：
2000-07-01
影响因子：
3.4
通讯作者：
Galla, HJ
中科院分区：
文献类型：
--
作者：
Bourdos, N;Kollmer, F;Galla, HJ
一种常用的含二棕榈酰磷脂酰胆碱（DPPC）、二棕榈酰磷脂酰甘油（DPPG）和表面活性蛋白C（SP - C）的肺表面活性剂模型，通过空间分辨飞行时间二次离子质谱（TOF - SIMS）作为朗缪尔 - 布洛杰特膜进行分析，以直接观察畴的形成和组成。对二元脂质和脂质/SP - C体系进行了探测以作比较。TOF - SIMS光谱显示出DPPC和SP - C的特征性正二次离子（S1），但没有DPPG的。S1成像结果在DPPC/DPPG和DPPG/SP - C中呈现出具有畴结构的图像，但在DPPC/SP - C膜中没有。我们能够区分流体区域和凝聚区域，可能是由于基质效应。这些发现与其他成像技术，如荧光光学显微镜（FLM）、扫描力显微镜（SFM）和银染法相符。从塌陷区域转移的三元混合物DPPC/DPPG/SP - C呈现出围绕纯脂质区域的富含SP - C的畴。所得结果与我们早期的扫描力显微镜（SFM）分层突起图像完全一致，这些分层突起在扩张过程中作为表面活性剂材料的压缩储存库。我们的研究再次表明SP - C在呼吸过程中起着独特的作用。
An often-used model lung surfactant containing dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and the surfactant protein C (SP-C) was analyzed as Langmuir-Blodgett film by spatially resolved time-of-flight secondary ion mass spectrometry (TOF-SIMS) to directly visualize the formation and composition of domains. Binary lipid and lipid/SP-C systems were probed for comparison. TOF-SIMS spectra revealed positive secondary ions (S1) characteristic for DPPC and SP-C, but not for DPPG. SI mapping results in images with domain structures in DPPC/DPPG and DPPG/SP-C, but not in DPPC/SP-C films. We are able to distinguish between the fluid and condensed areas probably due to a matrix effect. These findings correspond with other imaging techniques, fluorescence light microscopy (FLM), scanning force microscopy (SFM), and silver decoration. The ternary mixture DPPC/DPPG/SP-C transferred from the collapse region exhibited SP-C-rich domains surrounding pure lipid areas. The results obtained are in full accordance with our earlier SFM picture of layered protrusions that serve as a compressed reservoir for surfactant material during expansion. Our study demonstrates once more that SP-C plays a unique role in the respiration process.