ISOLATION OF TOXOPLASMA GONDII FROM ANIMALS IN DURANGO, MEXICO
ISOLATION OF TOXOPLASMA GONDII FROM ANIMALS IN DURANGO, MEXICO
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DOI：
10.1645/ge-1874.1
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发表时间：
2009-04-01
影响因子：
1.3
通讯作者：
Su, C.
中科院分区：
文献类型：
--
作者：
Dubey, J. P.;Velmurugan, G. V.;Su, C.
关于墨西哥农村人和动物弓形虫感染的流行病学知之甚少。150只犬（Canis familaris）、150只猫（Felis卡图斯）的血清样本和组织。65负鼠（Didelphis virginianus）。249只大鼠（鼠属）。对杜兰戈地区的127只小鼠（小家鼠）和69只松鼠（黄鼠）进行了T.弓形虫感染使用改良的凝集试验和1：25的血清稀释度，在68（45.3%）的150只狗中发现了这种寄生虫的抗体。150只猫中14只（9.3%），66只负鼠中11只（16.6%），249只大鼠中2只（0.8%），127只小鼠中4只（3.1%），69只松鼠中0只。对狗、猫、负鼠、大鼠、小鼠和松鼠的组织（脑和心脏）进行生物测定，以确定T。刚地。维伯河从28只血清反应阳性的狗中的3只和8只血清反应阳性的猫中的5只的组织中分离到弓形虫，但从其它动物中未分离到弓形虫。从3 T.对来自狗的弓形虫分离株、来自5只猫的6个分离株和来自墨西哥的自由放养鸡的4个分离株（先前分离株）进行基因分型。PCR-RFLP分型采用B1、SAG 1、SAG 2、SAG 3、BTUB、GRA 6、c22-8、c29-2、L358. PKI和Apico）鉴定出5种基因型。一种基因型（4个鸡分离株）属于克隆III型谱系，在以前的报告中报告了三种基因型，其中一种基因型是独特的。
Little is known concerning the epidemiology of Toxoplasma gondii infection in people and animal in rural Mexico. Serum samples and tissues from 150 dogs (Canis familaris), 150 cats (Felis catus). 65 opossums (Didelphis virginianus). 249 rats (Rattus spp.). 127 mice (Mus musculus), and 69 squirrels (Spermophilus variegatus) from the Durango area were evaluated for T. gondii infection. Using a modified agglutination test and a serum dilution of 1:25, antibodies to this parasite were found in 68 (45.3%) of 150 dogs. 14 (9.3%) of 150 cats, 11 (16.6%) of 66 opossums, 2 (0.8%) of 249 rats, 4 (3.1%) of 127 mice, and 0 of 69 squirrels. Tissues (brain and heart) of dogs, cats, opossums, rats, mice, and squirrels were bioassayed in mice for the presence of T. gondii. Viable T. gondii was isolated in tissues from 3 of 28 seropositive dogs and 5 of 8 seropositive cats, but not from the other animals. The DNA obtained from the 3 T. gondii isolates from dogs, 6 isolates from 5 cats, and 4 isolates from free-range chickens from Mexico, previously isolate, were genotyped. The PCR-RFLP typing, which used 11 markers (B1, SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358. PKI, and Apico), identified 5 genotypes. One genotype (the 4 chicken isolates) belongs to the clonal Type III lineage, three genotypes were reported in previous reports, and 1 genotype is unique.