Molecular biology of the anion exchanger gene family.
Molecular biology of the anion exchanger gene family.
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DOI：
10.1016/s0074-7696(08)60674-9
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
Kopito, R R
中科院分区：
文献类型：
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作者：
Kopito, R R
阴离子交换器基因家族至少由四到五个成员组成，其中三个成员已经在cdna水平上得到了鉴定。AE1-3编码的多肽与红细胞阴离子交换带3(AE1)有显著的同源性。编码这些基因的cDNA在异源系统中的表达证实了这种序列相似性反映在介导可逆的Cl/HCO3交换的能力上。虽然已知带3的NH2末端结构域与红细胞中的几种细胞质蛋白相互作用，但AE2和AE3类似结构域的功能尚不清楚。在鸟类和哺乳动物的红系祖细胞中，AE1基因与其他红系基因在红细胞生成过程中协同表达。此外，AE1在肾脏分泌酸性的间质细胞的基侧质膜上表达。AE2在许多上皮和非上皮细胞中表达；它可能在其中一些细胞的高尔基体中表达。AE3在兴奋性组织中表达，包括神经元和肌肉。这些蛋白质很可能在调节细胞内的pH值和各自组织中的氯化物方面发挥作用。了解这些蛋白质在离子运输和质膜组织中的生理作用仍然是一个中心问题。
The gene family of anion exchangers consists of at least four or five members, of which three have been characterized at the cDNA level. AE1-3 encode polypeptides that share significant homology with the erythrocyte anion exchanger, band 3 (AE1). Expression of cDNAs encoding these genes in heterologous systems confirms that this sequence similarity is reflected in the capacity to mediate reversible Cl/HCO3 exchange. While the NH2-terminal domain of band 3 is known to interact with several cytoplasmic proteins in erythrocytes, the function of the analogous domains of AE2 and AE3 remains unknown. The AE1 gene is expressed coordinately with other erythroid genes during erythropoiesis in both avian and mammalian erythroid progenitor cells. In addition, AE1 is expressed at the basolateral plasma membrane of the acid-secreting intercalated cells of the kidney. AE2 is expressed in a number of epithelial and nonepithelial cells; it may be expressed in the Golgi apparatus of some of these cells. AE3 is expressed in excitable tissues, including neurons and muscle. It is likely that these proteins play a role in regulation of intracellular pH and chloride in their respective tissue. Understanding of the physiological roles of these proteins, both for ion transport and for plasma membrane organization, remains a central issue.