LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
复制标题
DOI：
10.1242/dev.02604
复制
发表时间：
2006-11-01
期刊：
影响因子：
4.6
通讯作者：
Chung, Won-Il
中科院分区：
文献类型：
--
作者：
Lee, Young Koung;Kim, Gyung-Tae;Chung, Won-Il
植物的叶形态多样化，以适应不同的生态位。然而，负责调节叶形态的分子组分尚未完全阐明。通过筛选拟南芥激活标签系，我们确定了一个显性突变体，我们命名为longifolia 1 -1D（Ing 1 -1D）。Ing 1 -1D植株叶柄长，叶片窄而极长，边缘锯齿状，叶片细长。口器官和长角果。突变体的伸长叶是由于极性细胞伸长增加而不是细胞增殖增加。分子特征表明，这种表型是由新基因LNG 1的过表达引起的，该基因在拟南芥中被发现具有同源物LNG 2。为了进一步研究LNG基因的作用，我们表征了Ing 1和Ing 2功能丧失突变株系。与Ing 1 -1D植物的伸长叶相反，Ing 1和Ing 2突变体显示出略微降低的叶长。此外，Ing 1 -3 Ing 2 -1双突变体表现出进一步降低的叶长与较少的纵向极细胞伸长。Ing 1 -3 Ing 2 -1突变体植株的叶宽与野生型相似，这意味着LNG 1和LNG 2对极细胞伸长的作用与ROTUNDIFOLIA 3（ROT 3）相似。然而，对Ing 1 -3 Ing 2 -1 rot 3 -1三重突变体和Ing 1 -1D rot 3 -1双突变体的分析表明，LNG 1和LNG 2独立于ROT 3促进纵向细胞伸长。总之，这些发现表明，LNG 1和LNG 2是新的组件，调节叶形态，积极促进纵向极性细胞伸长独立的ROT 3在拟南芥。
Plants have diversified their leaf morphologies to adapt to diverse ecological niches. The molecular components responsible for regulating leaf morphology, however, have not been fully elucidated. By screening Arabidopsis activation-tagging lines, we identified a dominant mutant, which we designated longifolia1-1D (Ing1-1D). Ing1-1D plants were characterized by long petioles, narrow but extremely long leaf blades with serrated margins, elongated. oral organs, and elongated siliques. The elongated leaves of the mutant were due to increased polar cell elongation rather than increased cell proliferation. Molecular characterization revealed that this phenotype was caused by overexpression of the novel gene LNG1, which was found to have a homolog, LNG2, in Arabidopsis. To further examine the role of the LNG genes, we characterized Ing1 and Ing2 loss-of-function mutant lines. In contrast to the elongated leaves of Ing1-1D plants, the Ing1 and Ing2 mutants showed slightly decreased leaf length. Furthermore, the Ing1-3 Ing2-1 double mutant showed further decreased leaf length associated with less longitudinal polar cell elongation. The leaf widths in Ing1-3 Ing2-1 mutant plants were similar to those in wild type, implying that the role of LNG1 and LNG2 on polar cell elongation is similar to that of ROTUNDIFOLIA3 (ROT3). However, analysis of a Ing1-3 Ing2-1 rot3-1 triple mutant and of a Ing1-1D rot3-1 double mutant indicated that LNG1 and LNG2 promote longitudinal cell elongation independently of ROT3. Taken together, these findings indicate that LNG1 and LNG2 are new components that regulate leaf morphology by positively promoting longitudinal polar cell elongation independently of ROT3 in Arabidopsis.