Cyclic expression of class I alcohol dehydrogenase in male rats treated with ethanol
Cyclic expression of class I alcohol dehydrogenase in male rats treated with ethanol
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DOI：
10.1006/bbrc.2000.3186
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发表时间：
2000-08-11
影响因子：
3.1
通讯作者：
Ingelman-Sundberg, M
中科院分区：
文献类型：
--
作者：
Badger, TM;Hoog, JO;Ingelman-Sundberg, M
Continuous infusion of ethanol-containing diets has been demonstrated to generate well-defined pulses in blood and urine ethanol concentrations that occur with a frequency of approximately 6 days. The present study aimed to determine if hepatic class I alcohol dehydrogenase was the cause of these cycles. Adult male rats were fed an ethanol-containing diet by continuous intragastric infusion. Hepatic ADH activity, class I ADH mRNA level and rate of class I ADH gene transcription fluctuated in a cyclic pattern that positively correlated with UECs, and inhibition of ADH with 4-methylpyrazole abolished the UEC pulses. These data demonstrate for the first time an ethanol-dependent regulation of rat hepatic class I ADH. The cyclic behavior of the ethanol levels correlates with changes in class I ADH expression and implies adaptability of the ethanol eliminating system to high concentrations of alcohol. (C) 2000 Academic Press.
