NuRD complex component Mi-2beta binds to and represses RORgamma-mediated transcriptional activation.
NuRD complex component Mi-2beta binds to and represses RORgamma-mediated transcriptional activation.
复制标题
NuRD 复合体成分 Mi-2beta 结合并抑制 RORgamma 介导的转录激活。
DOI：
10.1016/j.bbrc.2004.04.087
复制
发表时间：
2004
影响因子：
3.1
通讯作者：
Chen,JDon
中科院分区：
文献类型：
--
作者：
Johnson,DavidR;Lovett,JeanneM;Hirsch,Michael;Xia,Fang;Chen,JDon
RORγ is a nuclear receptor that binds to DNA motifs as a monomer to constitutively activate target genes. RORγ plays an important role in thymocyte development and lymph node organogenesis, while the regulation of RORγ-mediated transcriptional activation is currently unclear. The purpose of this study was to identify other nuclear proteins that interact with RORγ. A yeast two-hybrid screen with Y190 yeast cells under stringent conditions resulted in the identification of CHD4, also known as Mi-2β, as a RORγ-interacting protein. This interaction was confirmed by GST pull-down assays. This interaction occurred within the middle regulatory region (amino acids 719–1164) of Mi-2β. Transfection of Gal4-RORγ into HeLa cells resulted in constitutive transactivation of the MH100-tk-luc reporter. The addition of Mi-2β resulted in a dramatic 50% decrease in Gal4-RORγ-mediated transactivation. These data demonstrate that RORγ forms a protein–protein interaction with the regulatory region of Mi-2β, resulting in inhibition of RORγ transcriptional activity. These results may provide evidence as to how RORγ-mediated transactivation is regulated by other nuclear proteins.
