Monolithic column with zwitterionic stationary phase for capillary electrochromatography
Monolithic column with zwitterionic stationary phase for capillary electrochromatography
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DOI：
10.1021/ac0496695
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发表时间：
2004-08-15
影响因子：
7.4
通讯作者：
Zou, HF
中科院分区：
文献类型：
--
作者：
Fu, HJ;Xie, CH;Zou, HF
以甲基丙烯酸丁酯、二甲基丙烯酸乙酯、甲基丙烯酸和甲基丙烯酸二甲氨基乙酯为原料，在致孔剂存在下进行原位聚合，制备了具有两性离子固定相的毛细管电色谱(CEC)整体柱。由于固定相含有两性离子官能团，即叔胺基团和丙烯酸基团，因此这些基团在两性离子固定相上的电离受流动相pH值的影响，进而影响电渗流的强度和方向。基于疏水机理，实现了烷基苯和多环芳烃的分离。在所制备的色谱柱上，采用阳极电渗滤法和阴极电渗滤法，在CEC模式下实现了苯酚、苯胺、多肽等多种极性化合物的分离，并用不同pH值的流动相在整体毛细管柱上观察到了不同的分离选择性。
A capillary electrochromatography (CEC) monolithic column with zwitterionic stationary phases was prepared by in situ polymerization of butyl methacrylate, ethylene dimethacrylate, methacrylic acid, and 2-(dimethyl amino) ethyl methacrylate in the presence of porogens. The stationary phases have zwitterionic functional groups, that is, both tertiary amine and acrylic acid groups, so the ionization of those groups on the zwitterionic stationary phase was affected by the pH values of the mobile phase, and further affects the strength and direction of the electroosmotic flow (EOF). Separations of alkylbenzenes and polycylic aromatic hydrocarbons based on the hydrophobic mechanism were obtained. Separation of various types of polar compounds, including phenols, anilines, and peptides, on the prepared column were performed under CEC mode with anodic and cathodic EOF, and different separation selectivities of those polar analytes were observed on the monolithic capillary column by using mobile phases with different pH values.