Connecting the transcription site to the nuclear pore: a multi-tether process that regulates gene expression
Connecting the transcription site to the nuclear pore: a multi-tether process that regulates gene expression
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DOI：
10.1242/jcs.053694
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发表时间：
2010-06-15
影响因子：
4
通讯作者：
Stutz, Francoise
中科院分区：
文献类型：
--
作者：
Dieppois, Guennaelle;Stutz, Francoise
基因在细胞核中的位置可以影响其活性水平，这一点现在已经得到了很好的证实。到目前为止，特别强调的是核膜（NE）作为一个转录沉默的核子域。然而，最近的工作表明，外周定位并不总是与阻遏，而是履行基因表达的双重功能。特别是，在酿酒酵母中，大量的高表达基因和激活的诱导基因优先与核孔复合物（NPC），这是一个由转录位点和NPC之间的瞬时相互作用介导的过程。最近的研究旨在揭示这种机制的分子基础，揭示了在NPC中基因的维持涉及基因表达的不同步骤的多个系链。这些观察结果与转录，mRNA加工和输出到细胞质之间的紧密联系一致，并强调了NPC在促进和协调基因表达过程中的作用。在这篇评论中，我们讨论了参与主动基因锚定到NPC的因素，以及NPC环境在促进基因表达中的各种新兴作用，重点是酵母作为模式生物。
It is now well established that the position of a gene within the nucleus can influence the level of its activity. So far, special emphasis has been placed on the nuclear envelope (NE) as a transcriptionally silent nuclear sub-domain. Recent work, however, indicates that peripheral localization is not always associated with repression, but rather fulfills a dual function in gene expression. In particular, in the yeast Saccharomyces cerevisiae, a large number of highly expressed genes and activated inducible genes preferentially associate with nuclear pore complexes (NPCs), a process that is mediated by transient interactions between the transcribed locus and the NPC. Recent studies aimed at unraveling the molecular basis of this mechanism have revealed that maintenance of genes at the NPC involves multiple tethers at different steps of gene expression. These observations are consistent with tight interconnections between transcription, mRNA processing and export into the cytoplasm, and highlight a role for the NPC in promoting and orchestrating the gene expression process. In this Commentary, we discuss the factors involved in active gene anchoring to the NPC and the diverse emerging roles of the NPC environment in promoting gene expression, focusing on yeast as a model organism.