Schistosoma haematobium: Identification of new estrogenic molecules with estradiol antagonistic activity and ability to inactivate estrogen receptor in mammalian cells
Schistosoma haematobium: Identification of new estrogenic molecules with estradiol antagonistic activity and ability to inactivate estrogen receptor in mammalian cells
复制标题
DOI：
10.1016/j.exppara.2010.06.012
复制
发表时间：
2010-12-01
影响因子：
2.1
通讯作者：
Correia da Costa, Jose Manuel
中科院分区：
文献类型：
--
作者：
Botelho, Monica Catarina;Soares, Raquel;Correia da Costa, Jose Manuel
我们已经鉴定了血吸虫总抗原(Sh)表达雌二醇(E_2)相关分子。我们现在证明，该分子在体外具有雌激素的拮抗作用。我们的结果与雌激素分子的存在是一致的，该分子可以拮抗雌二醇的活性。我们发现了这种分子的证据，因为我们鉴定和表征了以前未知的新的雌激素分子，存在于血吸虫虫体提取物和血吸虫感染者的血清中。我们还证明了Sh在体外能够与雌激素受体(ER)相互作用，解释了宿主内分泌系统如何有利于血吸虫的建立。这些发现突出了血吸虫对宿主内分泌系统的利用，并代表了血吸虫发育的另一个调节成分，它定义了一种新的范式，使宿主-寄生虫相互作用。这些分子的鉴定为开发治疗血吸虫病的替代药物开辟了新的途径。(C)2010 Elsevier Inc.保留所有权利。
We have previously identified the expression of an estradiol (E2)-related molecule by Schistosoma haematobium total antigen (Sh). We now show that this molecule has an antagonistic effect of estradiol in vitro. Our results are consistent with the existence of an estrogenic molecule that antagonizes the activity of estradiol. We found evidence for this molecule as we identified and characterized by mass spectrometry new estrogenic molecules previously unknown, present in schistosome worm extracts and sera of Schistosoma-infected individuals. We also show that Sh is able to interact in vitro with estrogen receptor (ER), explaining how host endocrine system can favor the establishment of schistosomes. These findings highlight the exploitation of the host endocrine system by schistosomes and represent an additional regulatory component of schistosome development that defines a novel paradigm enabling host-parasite interactions. The identification of these molecules opens new ways for the development of alternative drugs to treat schistosomiasis. (C) 2010 Elsevier Inc. All rights reserved.