Toward Larger DNA Origami
Toward Larger DNA Origami
复制标题
DOI：
10.1021/nl502626s
复制
发表时间：
2014-10-01
期刊：
影响因子：
10.8
通讯作者：
LaBean, Thomas H.
中科院分区：
文献类型：
--
作者：
Marchi, Alexandria N.;Saaem, Ishtiaq;LaBean, Thomas H.
结构 DNA 纳米技术，特别是支架式 DNA 折纸技术，正在迅速发展成为自下而上制造新型纳米级材料和设备的通用方法。然而，传统单链支架的长度，例如来自 M13mp18 噬菌体的 7,249 核苷酸环状基因组 DNA，限制了这些独特可寻址结构的规模。此外，增加 DNA 折纸尺寸会增加主链合成的成本负担。我们通过开发以下方法解决了这个双重问题：(1) 使用 lambda/M13 杂交病毒生产迄今为止最大的生物衍生单链支架，以产生环状单链形式的 51 466 核苷酸 DNA；(2) 通过喷墨打印工艺在压花有由环烯烃共聚物制成的功能化微柱的芯片上进行廉价的 DNA 合成。我们通过实验证明，可以非常有效地从由芯片衍生的短纤维链折叠的 lambda/M13 混合支架组装出 51 千碱基对折纸。此外，我们还展示了具有受控全局曲率的二维不对称折纸片，使得它们以可预测的方向降落在基板上，这已经通过原子力显微镜验证。
Structural DNA nanotechnology, and specifically scaffolded DNA origami, is rapidly developing as a versatile method for bottom-up fabrication of novel nanometer-scale materials and devices. However, lengths of conventional single-stranded scaffolds, for example, 7,249-nucleotide circular genomic DNA from the M13mp18 phage, limit the scales of these uniquely addressable structures. Additionally, increasing DNA origami size generates the cost burden of increased staple-strand synthesis. We addressed this 2-fold problem by developing the following methods: (1) production of the largest to-date biologically derived single-stranded scaffold using a lambda/M13 hybrid virus to produce a 51 466-nucleotide DNA in a circular, single-stranded form and (2) inexpensive DNA synthesis via an inkjet-printing process on a chip embossed with functionalized micropillars made from cyclic olefin copolymer. We have experimentally demonstrated very efficient assembly of a 51-kilobasepair origami from the lambda/M13 hybrid scaffold folded by chip-derived staple strands. In addition, we have demonstrated two-dimensional, asymmetric origami sheets with controlled global curvature such that they land on a substrate in predictable orientations that have been verified by atomic force microscopy.