Remodeling of myofibrils: subcellular distribution of myosin heavy chain mRNA and protein.
Remodeling of myofibrils: subcellular distribution of myosin heavy chain mRNA and protein.
复制标题
肌原纤维的重塑：肌球蛋白重链 mRNA 和蛋白质的亚细胞分布。
DOI：
10.1152/ajpregu.1992.262.3.r339
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Goldspink,PH
中科院分区：
文献类型：
--
作者：
Russell,B;Wenderoth,MP;Goldspink,PH
肌原纤维重塑发生在正常生活、生长和发育过程中，也发生在异构体转换发生的特殊情况下。在这篇综述中，我们集中在肌凝蛋白如何被纳入a带的超微结构方面。解剖方法的研究强调包括同位素和免疫化学标记以及原位杂交。我们得出结论，重塑的机制是单体肌球蛋白池与粗丝之间持续有序的交换。肌球蛋白mRNA的分布是肌纤维间和非肌共聚的，这表明新翻译的肌球蛋白在扩散和插入肌纤维晶格的a带之前被释放。
Myofibril remodeling occurs during the normal life, in growth and development, and in the special case when isoform switching occurs. In this review we concentrate on the ultrastructural aspects of how myosin is incorporated into the A-band. Anatomic methods of study are emphasized that include isotope and immunochemical labeling as well as in situ hybridization. We conclude that the mechanism of remodeling is one of continual orderly exchange between a monomeric myosin pool and the thick filament. Myosin mRNA distribution is intermyofibrillar and nonsarcomeric, which suggests that newly translated myosin is released before diffusion and insertion in the A-band of the myofibrillar lattice.