Ultrasensitive chemiluminescence of tetracyclines in the presence of MCLA
Ultrasensitive chemiluminescence of tetracyclines in the presence of MCLA
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DOI：
10.1016/j.jlumin.2017.02.017
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发表时间：
2017-06
影响因子：
3.6
通讯作者：
Wangsheng Zeng;Chenyao Zhu;Hongcheng Liu;Jing Liu;Hongping Cai;Xianglei Cheng;Lijun Wei
中科院分区：
文献类型：
--
作者：
Wangsheng Zeng;Chenyao Zhu;Hongcheng Liu;Jing Liu;Hongping Cai;Xianglei Cheng;Lijun Wei
本文报道了甲氧基化Cypridinaluciferin类似物（MCLA）与Ce（IV）的化学发光新体系，并对5种四环素类抗生素进行了化学发光分析。结果表明，加入10 pmol金霉素可使MCLA-Ce（IV）体系的化学发光强度增强30倍。通过化学发光光谱、自由基清除剂和紫外吸收光谱研究了MCLA-Ce（IV）-TCs的作用机理。结果表明，Ce（IV）与TC反应产生的单线态氧参与了CL现象的增强。建立了流动注射-化学发光联用分析系统，用于五种常见三氯甲烷的测定。线性范围为100 fmol至20 pmol（r>0.999），检测限为20至50 fmol（S/N=3）。本方法的灵敏度与UPLC-MS/MS检测TC的灵敏度相当。
In this article, five tetracyclines (TCs) showed ultrasensitive chemiluminescence (CL) based on the novel CL system of methoxylatedCypridinaluciferin analogues (MCLA) and Ce(IV). It was found that the CL intensity of MCLA-Ce(IV) system was enhanced 30 times by adding 10 pmol chlortetracycline. Mechanisms of MCLA-Ce(IV)-TCs were investigated by CL spectra, radical scavengers and UV spectra. The results indicated that singlet oxygen, resulting from the reaction between Ce(IV) and TCs, was involved in the enhancement of CL phenomenon. This CL system coupling with flow injection analysis was developed for the determination of five common TCs. Linear ranged from 100 fmol to 20 pmol (r>0.999) with limit of detection of 20 to 50 fmol (S/N=3). The sensitivity of the present method is comparable to that of UPLC-MS/MS detection for TCs.