Antiproliferative activity is present in bladder but not renal pelvic urine from interstitial cystitis patients
Antiproliferative activity is present in bladder but not renal pelvic urine from interstitial cystitis patients
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DOI：
10.1016/s0022-5347(05)68345-0
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发表时间：
1999-10-01
影响因子：
6.6
通讯作者：
Whitmore, KE
中科院分区：
文献类型：
--
作者：
Keay, S;Warren, JW;Whitmore, KE
目的：为了确定是否有抗增殖尿因子，我们以前发现是特异性的尿间质性膀胱炎（IC）患者起源于下尿路或更近端的situation.Materials和方法：连续导尿管尿液标本收集在无菌条件下从膀胱和肾盂的20 IC患者和一个对照患者（压力性尿失禁）。抗增殖活性通过用pH和渗透压校正的膀胱或输尿管尿液标本培养的原代正常成人膀胱上皮细胞的H-3-胸苷掺入来测定;显著抑制定义为H-3-胸苷掺入的变化大于对照细胞平均值的2个标准偏差。20例IC患者中有19例的膀胱尿标本与单独的细胞培养基相比显著抑制H-3-胸苷掺入（膀胱标本的平均变化= -68.7 +/-7.5%），而20例IC患者中只有1例的肾盂标本显著抑制增殖（肾盂标本的平均变化= 3.2 +/-3.4%）（p
Purpose: To determine whether an antiproliferative urine factor that we previously discovered to be specific for urine from interstitial cystitis (IC) patients originated in the lower urinary tract or a more proximal site.Materials and Methods: Sequential catheterized urine specimens were collected under sterile conditions from the bladder and renal pelvis of 20 IC patients and one control patient (with stress incontinence). Antiproliferative activity was determined by H-3-thymidine incorporation of primary normal adult bladder epithelial cells cultured with pH- and osmolality-corrected bladder or ureteral urine specimens; significant inhibition was defined as a change in H-3-thymidine incorporation greater than 2 standard deviations from the mean of control cells.Results: Bladder urine specimens from 19 of 20 IC patients significantly inhibited H-3-thymidine incorporation as compared to cell medium alone (mean change for bladder specimens = -68.7 +/- 7.5%), while a renal pelvic specimen from only 1 of 20 IC patients inhibited proliferation significantly (mean change for renal pelvic specimens = 3.2 +/- 3.4%) (p