Mechanoscopy: A Novel Device and Procedure for in vivo Detection of Chronic Colitis in Mice.
Mechanoscopy: A Novel Device and Procedure for in vivo Detection of Chronic Colitis in Mice.
复制标题
机械镜检查：一种用于体内检测小鼠慢性结肠炎的新型装置和程序。
DOI：
10.1093/ibd/izac046
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Saeidi,Nima
中科院分区：
文献类型：
--
作者：
He,Shijie;Azar,DaraA;Esfahani,FaridNasr;Azar,GolaraA;Shazly,Tarek;Saeidi,Nima
BackgroundGut stiffening caused by fibrosis plays a critical role in the progression of inflammatory bowel disease (IBD) and colon cancer. Previous studies have characterized the biomechanical response of healthy and pathological gut, with most measurements obtainedex vivo.MethodsHere, we developed a device and accompanying procedure forin vivoquantification of gut stiffness, termedmechanoscopy. Mechanoscopy includes a flexible balloon catheter, pressure sensor, syringe pump, and control system. The control system activates the balloon catheter and performs automated measurements of the gut stress-strain biomechanical response.ResultsA gut stiffness index (GSI) is identified based on the slope of the obtained stress-strain response. Using a colitis mouse model, we demonstrated that GSI positively correlates with the extent of gut fibrosis, the severity of mucosal damage, and the infiltration of immune cells. Furthermore, a critical strain value is suggested, and GSI efficiently detects pathological gut fibrotic stiffening when the strain exceeds this value.ConclusionsBased on these results, we envision that mechanoscopy and GSI will facilitate the clinical diagnosis of IBD.
