The effects of magnesium sulfate on blood-brain barrier disruption caused by intracarotid injection of hyperosmolar mannitol in rats
The effects of magnesium sulfate on blood-brain barrier disruption caused by intracarotid injection of hyperosmolar mannitol in rats
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DOI：
10.1016/j.lfs.2004.07.012
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发表时间：
2004-11-26
期刊：
影响因子：
6.1
通讯作者：
Sivas, A
中科院分区：
文献类型：
--
作者：
Kaya, M;Gulturk, S;Sivas, A
本研究旨在探讨硫酸镁是否能改变高渗甘露醇对血脑屏障（BBB）的破坏程度。Wistar成年雌性大鼠经左颈内动脉灌注25%甘露醇。每只动物腹膜内接受300 mg/kg负荷剂量的硫酸镁，溶于0.9%盐水中，然后再接受100 mg/kg剂量。在另一组中，在甘露醇给药前10 min，以150 mg/kg的剂量进行颈动脉内输注硫酸镁。伊文思蓝（EB）染料被用作BBB破坏的标志物。甘露醇和/或镁剂治疗后血糖和镁水平较治疗前升高（P < 0.01）。高渗甘露醇组左半球含水量显著增加（P < 0.01）。经镁处理后，灌流甘露醇的脑半球含水量明显减少（P < 0.05）。甘露醇灌流侧大脑半球EB染料含量明显高于同侧大脑半球（P < 0.01）。腹腔内和动脉内注射镁后，甘露醇灌注的半球中EB染料含量均比单独甘露醇灌注的半球中EB染料含量降低（P < 0.01）。我们的结论是，虽然硫酸镁管理颈动脉和腹膜内途径减弱血脑屏障破坏引起的高渗甘露醇，特别是腹膜内途径的硫酸镁管理可能提供一个有用的策略，以限制短暂的渗透开放血脑屏障。(C)2004年爱思唯尔公司All rights reserved.
The study was performed to evaluate whether magnesium sulfate could alter the degree of disruption of the blood-brain barrier (BBB) caused by hyperosmotic mannitol. Wistar adult female rats were infused with 25% mannitol into the left internal carotid artery. Each animal received intraperitoneally a 300 mg/kg loading dose of magnesium sulfate, dissolved in 0.9% saline, followed by a further 100 mg/kg dose. In the other group, intracarotid infusion of magnesium sulfate was performed at a dose of 150 mg/kg 10 min before mannitol administration. Evans blue (EB) dye was used as a marker of BBB disruption. The measured serum glucose and magnesium levels increased after mannitol and/or magnesium administration when compared with their initial values before treatment (P < 0.01). Water content of the left hemisphere was significantly increased by hyperosmotic mannitol (P < 0.01). The increased water content in the mannitol-perfused hemisphere was significantly decreased by magnesium treatment (P < 0.05). The content of EB dye in the mannitol-perfused hemisphere markedly increased when compared with the right hemisphere of the same brain (P < 0.01). The EB dye content in the mannitol-perfused hemisphere following both intraperitoneal and intraarterial administration of magnesium decreased when compared with mannitol alone (P < 0.01). We conclude that although magnesium sulfate administration by both intracarotid arterial and intraperitoneal routes attenuates BBB disruption caused by hyperosmolar mannitol, particularly intraperitoneal route of magnesium sulfate administration may provide a useful strategy to limit the transient osmotic opening of the BBB. (C) 2004 Elsevier Inc. All rights reserved.