Alternative Recruitment Strategies Influence Saliva Sample Return Rates in Community-Based Genetic Association Studies
Alternative Recruitment Strategies Influence Saliva Sample Return Rates in Community-Based Genetic Association Studies
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DOI：
10.1111/ahg.12009
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发表时间：
2013-05-01
影响因子：
1.9
通讯作者：
Furniss, Dominic
中科院分区：
文献类型：
--
作者：
Bhutta, Mahmood F.;Hobson, Lindsey;Furniss, Dominic
收集唾液进行DNA提取为从社区招募参与者进行遗传关联研究创造了新的机会。然而，样本回报率是可变的。之前没有研究专门讨论研究设计如何影响样本返回。使用三个大规模的遗传关联研究的数据，我们比较了招聘策略和样本回报率。我们发现研究之间的样本回报率存在非常显著的差异。在回顾性招募的研究中，在研究中心或家访提供样本的自限性疾病家庭的总体回报率远低于通过邮寄提供样本的慢性病成年老年人（59%对84%）。前瞻性招募与参与的高同意率（72%）相关，但随后实际唾液样本的回报率较低（42%）。在一项回顾性家庭研究中，电话对招募的影响很小，但在一项前瞻性家庭研究中，电话显著提高了回报。我们发现观察到的与未观察到的样本收集相比，或男性和女性成年参与者之间的DNA产量没有影响。总体而言，研究设计显著影响从社区招募的遗传关联研究的应答率。我们的研究结果将有助于研究人员在构建和成本的招聘协议。
Collection of saliva for DNA extraction has created new opportunities to recruit participants from the community for genetic association studies. However, sample return rates are variable. No prior study has specifically addressed how study design impacts sample return. Using data from three large-scale genetic association studies we compared recruitment strategy and sample return rates. We found highly significant differences in sample return rates between the studies. In studies that recruited retrospectively, overall returns were much lower from families with a self-limiting condition who provided samples at a research centre or home visit, than adult elderly individuals with a chronic disease who provided samples by post (59% vs. 84%). Prospective recruitment was associated with high agreement to participate (72%), but subsequent low return of actual saliva samples (42%). A telephone call had marginal effect on recruitment in a retrospective family study, but significantly improved returns in a prospective family study. We found no effect upon DNA yield comparing observed versus unobserved sample collection, or between male and female adult participants. Overall, study design significantly impacts upon response rates for genetic association studies recruiting from the community. Our findings will help researchers in constructing and costing a recruitment protocol.