HIV-1-specific CD4 helper function in persons with chronic HIV-1 infection on antiviral drug therapy as measured by ELISPOT after treatment with an inactivated, gp120-depleted HIV-1 in incomplete Freund's adjuvant.
HIV-1-specific CD4 helper function in persons with chronic HIV-1 infection on antiviral drug therapy as measured by ELISPOT after treatment with an inactivated, gp120-depleted HIV-1 in incomplete Freund's adjuvant.
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在接受抗病毒药物治疗的慢性 HIV-1 感染者中，在使用不完全弗氏佐剂中的灭活、gp120 耗尽的 HIV-1 治疗后，通过 ELISPOT 测量了 HIV-1 特异性 CD4 辅助功能。
DOI：
10.1097/00126334-200007010-00012
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
D. Carlo
中科院分区：
文献类型：
--
作者：
R. Moss;Erin Webb;W. Giermakowska;F. Jensen;J. Savary;M. Wallace;D. Carlo
目的：我们假设hiv -1血清阳性研究对象接受有效的抗病毒治疗和hiv特异性免疫治疗可以增加hiv -1特异性t辅助免疫功能。10名HIV-1血清阳性的研究受试者接受抗逆转录病毒治疗，在基线、第12周和第24周使用灭活的gp120缺失的IFA免疫原（HIV-1免疫原，remee）治疗。方法采用ELISPOT法检测HIV-1抗原刺激干扰素γ (ifn - γ)产生细胞的频率。结果研究对象感染hiv -1的频率显著增加（p < 0.05）。001)或p24抗原刺激（p < 0.01）的ifn - γ产生细胞经过1、2和3次HIV-1免疫原治疗。CD4细胞的消耗导致ifn - γ反应的最强消除。HIV-1的频率（r = 0.64; p = 0.0002）和p24的频率（r = 0.0001）。72年;p < 0.001)治疗前后cd8缺失人群中抗原刺激的ifn - γ产生细胞与淋巴细胞增殖反应相关。结论：HIV-1免疫原治疗显著提高了HIV-1特异性ifn - γ产生细胞的频率。目前正在进行研究，以确定艾滋病毒特异性能量的逆转与病毒学结果之间的关系。
OBJECTIVE We hypothesized that treatment of HIV-1-seropositive study subjects receiving potent antiviral therapy with an HIV-specific immune-based therapy would increase HIV-1-specific T-helper immune function. DESIGN 10 HIV-1-seropositive study subjects receiving antiretroviral therapy were treated with an inactivated, gp120-depleted immunogen in IFA (HIV-1 immunogen, Remune) at baseline, week 12, and week 24. METHODS The frequency of HIV-1 antigen-stimulated interferon-gamma (IFN-gamma)-producing cells was determined by the ELISPOT assay. RESULTS Study subjects significantly increased their frequency of HIV-1-stimulated (p <. 001) or p24 antigen-stimulated (p <.01) IFN-gamma-producing cells after one, two, and three treatments of HIV-1 immunogen. Depletion of CD4 cells resulted in the strongest abrogation of the IFN-gamma response. The frequency of HIV-1 (r = 0.64; p =.0002) and p24 (r = 0. 72; p <.001) antigen-stimulated IFN-gamma-producing cells in the CD8-depleted population before and after treatment was associated with the lymphocyte-proliferative response. CONCLUSIONS Treatment with HIV-1 immunogen significantly enhanced the frequency of HIV-1-specific IFN-gamma-producing cells. Studies are ongoing to determine the relationship between this reversal of HIV-specific anergy and virologic outcomes.
影响因子：
158.5
作者：
Zhang, LQ;Ramratnam, B;Ho, DD
通讯作者：
Ho, DD
影响因子：
6.4
作者：
Lederman, MM;Connick, E;Kessler, H
通讯作者：
Kessler, H