Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment.
Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment.
复制标题
开发皮肤印迹定量分析的改进方法：提高皮肤评估的可靠性和适用性。
DOI：
10.1111/ics.12217
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发表时间：
2015
影响因子：
2.3
通讯作者：
Sugama J and Sanada H
中科院分区：
文献类型：
--
作者：
Ogai K;Matsumoto M;Minematsu H;Kitamura K;Kobayashi M;Sugama J and Sanada H
最近开发了一种名为“皮肤印迹”的新型皮肤评估工具，可以轻松预测皮肤状态，以避免其恶化。本研究的目的是提出一种归一化方法，皮肤印迹，以弥补个体差异，可以妨碍定量比较和临床应用。MethodsTo normalize个体差异，我们利用了总蛋白作为一个“normalizer”与校准曲线。为了进行评价，我们进行了一项简单的模拟实验，其中以不同体积应用相同浓度的目标蛋白质[肿瘤坏死因子（TNF）-α]作为虚拟个体差异。此外，为了证明这种标准化的适用性，男性志愿者被招募皮肤印迹，然后估计TNF-α与normalization.ResultsWe获得了良好的校准曲线总蛋白（R2= 0.995）和TNF-α（R2= 0.997），这两者都是必要的一个精确的定量。在模拟实验中，我们估计了TNF-α的确切浓度，而不考虑应用体积，证明了这种归一化方法在皮肤印迹中的适用性。此外，对人类受试者的皮肤印迹显示了广泛的变化的总蛋白质含量，虽然正常化被认为是减少这种个别variation.ConclusionThis研究提出了总蛋白质正常化皮肤印迹与校准曲线。该方法可以加强皮肤印迹的定量性能，这可以扩展该方法作为皮肤评估工具在更广泛领域（例如护理和美容）中的适用性。
ObjectiveA novel skin assessment tool named ‘skin blotting’ has been recently developed, which can easily predict the skin status to avoid its deterioration. The aim of this study was to propose a normalization method for skin blotting to compensate for individual differences that can hamper the quantitative comparisons and clinical applications.MethodsTo normalize individual differences, we utilized a total protein as a ‘normalizer’ with calibration curves. For evaluation, we performed a simple simulation experiment, in which the same concentration of a protein of interest [tumour necrosis factor (TNF)‐α] was applied at different volumes as a virtual individual difference. Moreover, to demonstrate the applicability of this normalization, male volunteers were recruited for skin blotting followed by the estimation of TNF‐αwith normalization.ResultsWe obtained good calibration curves for total protein (R2= 0.995) and TNF‐α(R2= 0.997), both of which were necessary for an exact quantification. In the simulation experiment, we estimated the exact concentration of TNF‐αregardless of the applied volume, demonstrating the applicability of this normalization method in skin blotting. Further, skin blotting on human subjects showed a wide range of variation in the total protein content, although the normalization was thought to reduce such individual variations.ConclusionThis study has proposed total protein normalization for skin blotting with calibration curves. This method may strengthen the quantitative performance of skin blotting, which may expand the applicability of this method as a skin assessment tool in broader fields, such as nursing and cosmetology.