LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.
LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.
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DOI：
10.1186/1756-0500-5-45
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发表时间：
2012-01-19
影响因子：
1.8
通讯作者：
Levy M
中科院分区：
文献类型：
--
作者：
Yaffe H;Buxdorf K;Shapira I;Ein-Gedi S;Moyal-Ben Zvi M;Fridman E;Moshelion M;Levy M
快速RNA提取通常使用商业试剂盒进行，这些试剂盒非常昂贵并且可能涉及有毒试剂。这些试剂盒中的大多数可以用于健康的植物组织，但不能从坏死的真菌感染的组织或真菌菌丝体中产生一致的高质量RNA。我们报告了一种快速和相对便宜的方法，从植物和真菌感染的组织，以及从昆虫和真菌，盐酸胍缓冲液和常见的DNA提取柱最初用于提取和纯化质粒和cosmetry的基础上总RNA提取的发展。所提出的方法可重复用于从多种植物物种中分离RNA。它也可以用于受感染的植物组织和真菌菌丝体，其通常不符合标准核酸提取程序。
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium. We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids. The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.