Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses.
Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses.
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节肢动物中RNA病毒前所未有的基因组多样性揭示了负义RNA病毒的祖先
DOI：
10.7554/elife.05378
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发表时间：
2015-01-29
期刊：
影响因子：
7.7
通讯作者：
Zhang YZ
中科院分区：
文献类型：
--
作者：
Li CX;Shi M;Tian JH;Lin XD;Kang YJ;Chen LJ;Qin XC;Xu J;Holmes EC;Zhang YZ
虽然节肢动物是重要的病毒载体，但节肢动物病毒的生物多样性以及节肢动物在病毒起源和进化中所起的作用尚不清楚。通过对70种节肢动物进行RNA测序，我们发现了112种新病毒，这些病毒似乎是大部分记录的负义RNA病毒遗传多样性的祖先，其中一些病毒也以内源性基因组拷贝的形式存在。有了这个大大丰富的多样性，我们发现，节肢动物包含的病毒属于主要病毒组的基础，包括脊椎动物特异性沙粒病毒，丝状病毒，汉坦病毒，流感病毒，狂犬病毒，副粘病毒。我们同样记录了节肢动物病毒基因组结构的显着多样性，包括一个假定的圆形形式，这为基因组组织的进化提供了新的线索。因此，节肢动物是病毒遗传多样性的主要储存库，并且可能是病毒进化的核心。DOI：http://dx.doi.org/10.7554/eLife.05378.001网站
Although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. Through RNA sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense RNA viruses, a number of which are also present as endogenous genomic copies. With this greatly enriched diversity we revealed that arthropods contain viruses that fall basal to major virus groups, including the vertebrate-specific arenaviruses, filoviruses, hantaviruses, influenza viruses, lyssaviruses, and paramyxoviruses. We similarly documented a remarkable diversity of genome structures in arthropod viruses, including a putative circular form, that sheds new light on the evolution of genome organization. Hence, arthropods are a major reservoir of viral genetic diversity and have likely been central to viral evolution. DOI: http://dx.doi.org/10.7554/eLife.05378.001