A DREB gene from the xero-halophyte Atriplex halimus is induced by osmotic but not ionic stress and shows distinct differences from glycophytic homologues
A DREB gene from the xero-halophyte Atriplex halimus is induced by osmotic but not ionic stress and shows distinct differences from glycophytic homologues
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DOI：
10.1007/s11240-010-9906-2
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发表时间：
2011-08
期刊：
影响因子：
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通讯作者：
A. Khedr;M. Serag;Mamdouh M. Nemat-Alla;Amina Z. Abo-Elnaga;R. Nada;W. P. Quick;G. Abogadallah
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文献类型：
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作者：
A. Khedr;M. Serag;Mamdouh M. Nemat-Alla;Amina Z. Abo-Elnaga;R. Nada;W. P. Quick;G. Abogadallah
研究胁迫诱导基因的调控可以了解盐生植物在非生物胁迫下维持生长和繁荣的机制。本研究探讨了盐胁迫下离子组分和渗透组分对脱水反应元件结合转录因子（DREB）的调控作用，DREB属于非生物胁迫诱导的AP 2/EREB组，调控许多启动子中含有DRE结合位点的胁迫诱导基因，DREB受盐胁迫的渗透组分的调控，而不受盐胁迫的离子组分的调控。DREB可能不参与NHX 1、SOS 1和H +-PPase等钠调控基因的调控。此外，由于DREB的诱导时间不同，DREB可能直接或间接参与CMO的调节。此外，DREB是盐（5倍）和干旱（2倍）条件下上调最多的基因，这加强了该基因在A中的重要性。耐盐性。此外，它在正常条件下的组成型表达也表明它参与其他生长和发育程序。A.盐生植物中的盐芥基因的组成型表达以及盐生植物和糖生植物之间蛋白质结构的差异是盐生植物产生盐芥的主要原因。
Studying the regulation of stress inducible genes can lead to understanding of the mechanisms by which halophytes maintain growth and thrive under abiotic stress. Verifying whether ionic or osmotic components of salt stress control the regulation of Dehydration Responsive Element- binding transcription factor (DREB) was investigated in the present study.DREBbelongs to AP2/EREB group that is induced under abiotic stress and regulates many stress inducible genes that contain DRE binding sites in their promoters.DREBinAtriplex halimuswas regulated by the osmotic component but not by the ionic one of salt stress. It seemed thatDREBwas not involved in the regulation of sodium manipulating genes likeNHX1,SOS1 or H+-PPase. Moreover,DREBcould be involved directly of indirectly inCMOregulation because of timing of induction. Also,DREBwas the most upregulated gene under salt (fivefold) and drought (twofold) conditions, which reinforced the importance of this gene inA. halimustolerance to stress. Moreover, its constitutive expression under normal conditions also indicated its involvement in other growth and developmental programs. The tolerance ofA. halimusand of halophytes, in general, could be attributed to the constitutive expression of its genes and differences in protein structures between halophytes and glycophytes.