The essential effector SCRE1 in Ustilaginoidea virens suppresses rice immunity via a small peptide region
The essential effector SCRE1 in Ustilaginoidea virens suppresses rice immunity via a small peptide region
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黑粉菌中的重要效应子 SCRE1 通过小肽区域抑制水稻免疫力
DOI：
10.1111/mpp.12894
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发表时间：
2020-02-22
影响因子：
4.9
通讯作者：
Sun, Wenxian
中科院分区：
文献类型：
--
作者：
Zhang, Nan;Yang, Jiyun;Sun, Wenxian
稻曲病是近年来在世界范围内流行的一种新的植物病害，由稻曲病菌引起。联合virens基因组编码许多推测的效应蛋白，基于对其他致病系统的研究，这些效应蛋白可能在真菌毒力中起重要作用。然而，目前关于单株U.病毒效应器。在这里，我们报告了我们的鉴定和分泌的富含半胱氨酸的蛋白SCRE 1，这是一个重要的毒力效应在U。绿色的当SCRE 1在稻瘟病菌中异源表达时，该蛋白在感染过程中被分泌并易位到植物细胞中。SCRE 1抑制非宿主植物本氏烟草（Nicotiana benthamiana）中的免疫相关过敏反应。水稻中SCRE 1的诱导表达还抑制模式触发免疫，并增强对水稻细菌和真菌病原体的疾病易感性。免疫抑制活性定位于含有重要的“半胱氨酸-脯氨酸-丙氨酸-精氨酸-丝氨酸”基序的小肽区域。此外，使用CRISPR/Cas9系统产生的scre 1敲除突变体在U.病毒对水稻的毒力，这在很大程度上得到了全长SCRE 1基因的补充。总的来说，这项研究表明，效应SCRE 1能够抑制宿主免疫，是U.绿色的
Summary The biotrophic fungal pathogen Ustilaginoidea virens causes rice false smut, a newly emerging plant disease that has become epidemic worldwide in recent years. The U. virens genome encodes many putative effector proteins that, based on the study of other pathosystems, could play an essential role in fungal virulence. However, few studies have been reported on virulence functions of individual U. virens effectors. Here, we report our identification and characterization of the secreted cysteine‐rich protein SCRE1, which is an essential virulence effector in U. virens. When SCRE1 was heterologously expressed in Magnaporthe oryzae, the protein was secreted and translocated into plant cells during infection. SCRE1 suppresses the immunity‐associated hypersensitive response in the nonhost plant Nicotiana benthamiana. Induced expression of SCRE1 in rice also inhibits pattern‐triggered immunity and enhances disease susceptibility to rice bacterial and fungal pathogens. The immunosuppressive activity is localized to a small peptide region that contains an important ‘cysteine‐proline‐alanine‐arginine‐serine’ motif. Furthermore, the scre1 knockout mutant generated using the CRISPR/Cas9 system is attenuated in U. virens virulence to rice, which is greatly complemented by the full‐length SCRE1 gene. Collectively, this study indicates that the effector SCRE1 is able to inhibit host immunity and is required for full virulence of U. virens.