Identification and spatiotemporal expression of gpr161 genes in zebrafish.
Identification and spatiotemporal expression of gpr161 genes in zebrafish.
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斑马鱼gpr161基因的鉴定和时空表达。
DOI：
10.1016/j.gene.2019.144303
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Zhonghua Zhao
中科院分区：
文献类型：
--
作者：
Min Wang;Ping Li;Hao Wang;Lina Dong;Changxing Wu;Zhonghua Zhao
G蛋白偶联受体161(GPR161)是一种纤毛孤儿。据报道，Hedgehog(HH)信号通路在脊椎动物Hedgehog(HH)信号通路中起关键作用，HH信号通路在后生动物中保守，在早期胚胎发育和成体代谢的动态平衡中发挥作用。然而，到目前为止，所有的GPR161功能研究都只在小鼠身上进行。HH机制研究的常用材料NIH3T3细胞中的敲打outgpr161没有发现任何明显的HH途径缺陷，这提出了GPR161在HH途径中的功能是否在脊椎动物系统中保守的问题。在这里，我们描述了两个斑马鱼gpr161同源物gpr161a和gpr161b的特性和时空表达。gpr161a被重新命名为gpr161，而egpr161b则是新发现的。整装原位杂交和定量聚合酶链式反应结果表明，gpr161aa最初以母体方式表达，而egpr161bis则不表达。虽然这两个gpr161在胚胎早期普遍表达，但它们都有组织特异性蓄积。gpr161在中枢神经系统(CNS)和近轴细胞中丰富，那里有丰富的HH反应细胞。Togethergpr161a在成鱼的肌肉和肠道中高表达。这些结果有力地表明了GPR161a在斑马鱼HH信号转导中的调节作用。gpr161b也在中枢神经系统中积累，但主要集中在神经管的中线，其表达模式与wnt5b在该区域的表达相似，表明其潜在的功能与WNT信号通路有关。有趣的是，我们还发现了gpr161在受精后24小时在后血岛(PBI)中的特异性积聚，这表明gpr161可能在斑马鱼的早期造血中发挥作用。我们的工作为揭示gpr161在脊椎动物中的不同功能提供了一个起点，并将为研究HH和WNT通路的机制以及早期造血提供线索。
G protein coupled Receptor 161 (GPR161) is a ciliary orphan GPCR. It is reported to play critical roles in regulating vertebrate Hedgehog (Hh) signaling pathway, that is conserved in metazoan and functions in earlier embryogenesis and homeostasis of adult metabolism. However, to date, all GPR161 functional studies were performed only in mouse. Knock outgpr161in NIH3T3 cell lines, the common material for Hh mechanism research, failed to give any obvious Hh pathway defects, raising the question that whether GPR161 functions in Hh pathway is conserved in vertebrate system. Here, we described the characterization and spatiotemporal expression of two zebrafishgpr161homologs,gpr161aandgpr161b.gpr161awas renamed of thegpr161previously identified, whilegpr161bwas novel identified. The whole-mountin situhybridization and quantitative PCR results showed thatgpr161ais initially expressed in maternal manner whilegpr161bis not. Although these twogpr161showed ubiquitously expressed at early embryonic stages, each of them had tissue specific accumulation.gpr161ais abundant in the central nervous system (CNS) and adaxial cells, where are rich of Hh responding cells. Togethergpr161awas highly expressed in muscle and intestine in adult fishes. These results strongly suggest the regulating roles of Gpr161 a in zebrafish Hh signal transduction.gpr161bwas also accumulated in the CNS but mainly at the midline in the neural tube, similar pattern aswnt5bexpression in such area, suggesting its potential function correlated with WNT signaling pathway. Interestingly, we also found the specific accumulation ofgpr161in posterior blood island (PBI) at 24 hours post fertilization (hpf), indicating thegpr161may play roles in early hematopoiesis in zebrafish. Our work provides a starting point to unveil the divergent functions ofgpr161in vertebrate and will shed light on the studies of mechanism of Hh and WNT pathways, as well as early hematopoiesis.
影响因子：
2.7
作者：
Zacharias WJ;Madison BB;Kretovich KE;Walton KD;Richards N;Udager AM;Li X;Gumucio DL
通讯作者：
Gumucio DL
G 蛋白偶联受体 Gpr161 对 Sonic Hedgehog 通路的基础抑制限制了髓母细胞瘤的发病机制。
影响因子：
8.8
作者：
Shimada,IsseiS;Hwang,Sun-Hee;Somatilaka,BandarigodaN;Wang,Xin;Skowron,Patryk;Kim,Jiwoong;Kim,Min;Shelton,JohnM;Rajaram,Veena;Xuan,Zhenyu;Taylor,MichaelD;Mukhopadhyay,Saikat
通讯作者：
Mukhopadhyay,Saikat