QTL analysis for flowering time in carnation (Dianthus caryophyllus L.)
QTL analysis for flowering time in carnation (Dianthus caryophyllus L.)
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DOI：
10.1016/j.scienta.2019.109053
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发表时间：
2020-02
影响因子：
4.3
通讯作者：
M. Yagi;K. Shirasawa;H. Hirakawa;S. Isobe;J. Matsuno;Y. Uno;K. Tanase;T. Onozaki;H. Yamaguchi
中科院分区：
文献类型：
--
作者：
M. Yagi;K. Shirasawa;H. Hirakawa;S. Isobe;J. Matsuno;Y. Uno;K. Tanase;T. Onozaki;H. Yamaguchi
开花期是康乃馨的重要性状之一。因为它决定了每年的产量。利用两个已建立的作图群体，研究了开花时间的数量性状基因座（QTL）的数量和效应。开花时间在两个F2群体和不同年份表现出较大的表型分离。在不同的群体和年份中，第10连锁群检测到一个主要的开花时间QTL，其效应解释了18.2%~ 22.5%的表型变异。我们开发了一个DNA标记，qD 1Flw 1-sc 43 -4，它位于检测到的开花时间QTL附近。利用qD 1Flw 1-sc 43 -4标记对晚花品种‘Light Pink Barbara’和早花品种‘Kaneainou 1 go’杂交F1群体的开花时间进行了区分和基因型分类。我们的研究结果表明，开花时间在康乃馨涉及几个遗传因素。在本研究中，我们确定了开花时间的主要因素之一，并开发了一个与主效QTL紧密连锁的DNA标记。
Flowering time is one of the most important traits in carnation (Dianthus caryophyllusL.) breeding because it decides the yearly yield. Two previously developed mapping populations were used to determine the number and effect of quantitative trait loci (QTLs) for flowering time. Flowering time showed large phenotypic segregation in two F2populations and in different years. Despite the different populations and different years, one major common QTL for flowering time was detected in linkage group 10 with the effect explaining from 18.2%–22.5% of the overall phenotypic variance. We developed a DNA marker, qD1Flw1-sc43-4, that was located close to the detected QTL for flowering time. We could distinguish the flowering time and categorize the genotypes of an F1population derived from a cross between late flowering ‘Light Pink Barbara’ and early flowering ‘Kaneainou 1 go’ using the qD1Flw1-sc43-4 marker. Our results suggest that flowering time in carnation involves several genetic factors. In this study, we identified one of the major factors for flowering time and developed a DNA marker that was tightly linked to the major QTL.