Prefabricated Flaps: Identification of Microcirculation Structure and Supercharging Technique Improving Survival Area
Prefabricated Flaps: Identification of Microcirculation Structure and Supercharging Technique Improving Survival Area
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DOI：
10.1055/s-0036-1593770
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发表时间：
2016-11
影响因子：
2.1
通讯作者：
Heng Xu;S. Feng;Yimeng Xia;Zvi Steinberger;W. Xi;Hongwei Fang;Zhiwei Li;Yixin Xie;Yixin Zhang
中科院分区：
文献类型：
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作者：
Heng Xu;S. Feng;Yimeng Xia;Zvi Steinberger;W. Xi;Hongwei Fang;Zhiwei Li;Yixin Xie;Yixin Zhang
摘要背景微循环是研究预制皮瓣存活时经常被忽视的一个重要因素。在目前的研究中，我们使用不同的预制技术来表征皮瓣内的微循环，目的是寻找一种有效的方法来提高皮瓣的存活面积。方法采用两期手术建立鼠腹部预制皮瓣模型。将所有大鼠随机分为6组（n = 10/组）：A组，预制股血管；B组，预制股动脉连接腹壁下浅静脉（SIEV）；C组，将腹壁下浅动脉（SIEA）与预制股静脉连接；D组与A组相似，合并预制SIEA； E组与A组相似，合并预制SIEV；F组为对照组，由腹壁下浅血管滋养的轴状皮瓣组成。通过宏观分析、近红外荧光成像（NIFI）和组织学评估皮瓣的存活面积、血流灌注面积和毛细血管密度。结果B组与C组、D组与E组的生存面积比较，差异无统计学意义。D组和E组的生存面积大于B组和C组。B组至E组的生存面积小于F组，大于a组。A ~ C组和D ~ f组的毛细血管密度差异无统计学意义。结论动脉和静脉增压均可提高预制皮瓣的存活面积。
Abstract Background Microcirculation is an important factor frequently overlooked when studying the survival of prefabricated flaps. In the current study, we use different prefabrication techniques for characterizing microcirculation within the flap, with the goal of finding an effective way to improve its survival area. Methods An abdominal prefabricated flap rodent model was created using a two-stage operation. All rats were randomly divided into six groups (n = 10/group): group A, prefabricated femoral vessels; group B, prefabricated femoral artery with a connected superficial inferior epigastric vein (SIEV); group C, connected superficial inferior epigastric artery (SIEA) with a prefabricated femoral vein; group D was similar to group A along with a prefabricated SIEA, and group E was similar to group A along with a prefabricated SIEV; and group F acted as a control group and consisted of an axial flap nourished by superficial inferior epigastric vessels. Flaps were assessed for survival area, blood perfusion area, and capillary density using macroscopic analysis, near-infrared fluorescence imaging (NIFI), and histology. Results The survival area was not significantly different when comparing groups B to C, and D to E. The survival area of groups D and E was larger than that of groups B and C. Groups B through E had a smaller survival area in comparison to group F and a larger survival area than group A. NIFI were consistent with the macroscopic outcomes. The capillary density was not significantly different between groups A to C and groups D to F. Conclusion Both arterial and venous supercharging could potentially improve the survival area of prefabricated flaps.