REGULATION OF ADIPOCYTE PRECURSOR DNA-SYNTHESIS BY ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS - INTERACTION WITH HEPARIN AND OTHER GROWTH-FACTORS
REGULATION OF ADIPOCYTE PRECURSOR DNA-SYNTHESIS BY ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS - INTERACTION WITH HEPARIN AND OTHER GROWTH-FACTORS
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DOI：
10.1677/joe.0.1370369
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发表时间：
1993-06-01
影响因子：
4
通讯作者：
GODDARD, C
中科院分区：
文献类型：
--
作者：
BUTTERWITH, SC;PEDDIE, CD;GODDARD, C
脂肪组织的发育依赖于成纤维细胞样脂肪细胞前体细胞的生长和分化。脂肪前体细胞的体外培养为鉴定潜在的增殖和分化调节因子提供了理想的系统。我们已经证明，酸性成纤维细胞生长因子（aFGF）和碱性成纤维细胞生长因子（bFGF）刺激鸡脂肪细胞前体DNA合成的剂量依赖性方式高达100 μ g aFGF/l和1 μ g bFGF/l的浓度。bFGF的作用是双相的，因此在与25 μ g bFGF/l孵育时，DNA合成与对照组无显著差异。在肝素存在的情况下，25 μ g bFGF/l对DNA合成的刺激是1 μ g bFGF/l浓度的1.6倍。在含有aFGF的孵育液中加入肝素，可将最大刺激DNA合成所需的浓度降低至1 μ g/l。与aFGF（1-100 μ g/l）联合胰岛素样生长因子-I（IGF-1）、血小板衍生生长因子、转化生长因子-α或转化生长因子-β 1（TGF-β 1）孵育的细胞显示出DNA合成的显著协同增加。当使用1 μ g bFGF/l时也是如此，但在25 μ g bFGF/l的浓度下，仅观察到IGF-1和TGF-β 1的协同作用。这些结果表明，碱性和酸性FGF都是脂肪细胞增生的潜在重要调节剂，并且它们的作用受细胞外基质成分和其他生长因子的存在调节。
The development of adipose tissue is dependent on the growth and differentiation of fibroblast-like adipocyte precursor cells. Culture of adipocyte precursor cells in vitro has provided an ideal system for identifying potential regulators of proliferation and differentiation. We have demonstrated that both acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) stimulate chicken adipocyte precursor DNA synthesis in a dose-dependent manner up to a concentration of 100 mug aFGF/l and 1 mug bFGF/l. The effect of bFGF was biphasic, so that in incubations with 25 mug bFGF/l, DNA synthesis was not significantly different from controls. In the presence of heparin, stimulation of DNA synthesis at 25 mug bFGF/l was 1.6-fold greater than at a concentration of 1 mug bFGF/l. Addition of heparin to incubations containing aFGF reduced the concentration required for maximum stimulation of DNA synthesis to 1 mug/l. Cells incubated with aFGF (1-100 mug/l) in combination with insulin-like growth factor-I (IGF-1), platelet-derived growth factor, transforming growth factor-alpha or transforming growth factor-beta1 (TGF-beta1) exhibited a marked synergistic increase in DNA synthesis. This was also the case when 1 mug bFGF/l was used, but at a concentration of 25 mug bFGF/l synergy was only seen with IGF-I and TGF-beta1. These results suggest that both basic and acidic FGF are potentially important regulators of adipocyte hyperplasia and that their effect is modulated by constituents of the extracellular matrix and the presence of other growth factors.