POSTNATAL MATURATION OF RABBIT RENAL COLLECTING DUCT .3. PEANUT LECTIN-BINDING INTERCALATED CELLS
POSTNATAL MATURATION OF RABBIT RENAL COLLECTING DUCT .3. PEANUT LECTIN-BINDING INTERCALATED CELLS
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DOI：
10.1152/ajprenal.1992.262.2.f199
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发表时间：
1992-02-01
影响因子：
--
通讯作者：
SCHWARTZ, GJ
中科院分区：
文献类型：
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作者：
SATLIN, LM;MATSUMOTO, T;SCHWARTZ, GJ
对兔皮质集合管中HCO3跨上皮运输的测量表明，只有在出生后第一个月，HCO3的净分泌才变得明显[F.M.Mehrgut，L.M.Satlin，and G.J.Schwartz，AmJ.Physiol.259(肾液电解质生理性。28)：F801-F808,1990年]。我们使用荧光探针和免疫细胞化学来追踪β-嵌入细胞的出生后功能发育，β-嵌入细胞是完全分化的CCD的HCO3分泌细胞。在整个成熟过程中，β-插层细胞通过选择性摄取对pH敏感的染料2‘，7’-bis(carboxyethyl)-5(6)-carboxyfluorescein，-A碱性细胞pH，与花生凝集素和单抗B63的顶端结合，以及其顶端氯-HCO3交换的功能能力(依赖于氯的细胞内碱负荷的挤出)来鉴定。与成熟段相比，新生MID-Ccd有较少的间质细胞，其特点是碱性细胞pH降低，顶端ClHCO3交换活性降低，PNA的顶端结合谱变短。所有年龄段都有证据表明基底侧氯电导和相似的缓冲能力。在新生儿外皮质，很少或没有与PNA或B63结合。一旦检测到完全分化的β细胞所特有的细胞表面抗原，功能研究就表明存在顶端的氯-HCO3交换，尽管减少了。因此，兔肾中分泌HCO3的嵌合细胞在出生后就有增殖和成熟；这些细胞的来源仍有待确定。
Measurements of transepithelial HCO3 transport in the rabbit cortical collecting duct (CCD) indicate that net HCO3 secretion becomes apparent only after the first month of life [F. M. Mehrgut, L. M. Satlin, and G. J. Schwartz, Am. J. Physiol. 259 (Renal Fluid Electrolyte Physiol. 28): F801-F808, 1990]. We used fluorescent probes and immunocytochemistry to trace the postnatal functional development of the beta-intercalated cell, the HCO3-secreting cell of the fully differentiated CCD. Throughout maturation, the beta-intercalated cell was empirically identified by its selective uptake of the pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein, an alkaline cell pH, apical binding to peanut agglutinin (PNA) and monoclonal antibody B63, and by its functional capacity for apical Cl-HCO3 exchange as manifested by Cl-dependent extrusion of an intracellular alkali load. Compared with the mature segment, the neonatal mid-CCD exhibited fewer intercalated cells, which were characterized by a less alkaline cell pH, reduced apical Cl-HCO3 exchange activity, and shorter apical binding profiles for PNA. There was evidence for basolateral Cl conductance and similar buffering capacity at all ages. In the neonatal outer cortex there was little or no binding to PNA or to B63. As soon as cell surface antigens characteristic of the fully differentiated beta-cell were detected, functional studies indicated the presence, albeit reduced, of apical Cl-HCO3 exchange. Thus there is postnatal proliferation and maturation of HCO3-secreting intercalated cells in the rabbit kidney; the origin of these cells remains to be determined.