Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules
Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules
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DOI：
10.1002/jbm.a.33074
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发表时间：
2011-07-01
影响因子：
4.9
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
--
作者：
Kawase, Tomoyuki;Tanaka, Takaaki;Yoshie, Hiromasa
人培养骨膜片是一种很有前途的自体骨移植材料，它是由小块切除的骨膜组织段（PTSs）在培养皿中进行简单的扩增培养而成的。然而，PTSs与培养皿表面的初始粘附力较弱，常常阻碍细胞生长并限制了制备的数量。为了纠正这一缺点并避免使用动物源性粘附生物分子，我们开发了一种新型的可生物降解的多孔聚l-乳酸（pPLLA）膜。新切除的PTSs与高多孔pPLLA膜结合良好，可能是由于在上表面存在半半球20-30 μ m直径的开口。全球基因表达分析表明，在pPLLA膜上培养的骨膜片上调了许多粘附分子的表达。成骨诱导刺激了这些细胞产生蛋白聚糖，并同时增强了它们在体外矿化过程中向膜深孔区域的扩张和渗透。这些发现表明，我们的pPLLA膜不仅促进了主要由吸附蛋白介导的初始粘附，而且还通过诱导内源性粘附分子在骨膜片培养中增强了生物粘附。因此，使用这种新型的pPLLA膜可以大大提高骨膜片的治疗效果。(C) 2011 Wiley期刊公司[J] .中国生物医学工程学报，2011,31(1):393 - 398。
Human cultured periosteal sheets, which are developed from small excised periosteum tissue segments (PTSs) in culture dishes by simple expansion culture, have been applied as a promising autologous osteogenic grafting material for periodontal regenerative therapy. However, the weak initial adhesion of PTSs to dish surfaces often hampers cellular outgrowth and limits the number of preparations. To correct this weakness and still avoid the use of animal-derived adhesion biomolecules, we have developed a novel, biodegradable, porous poly(L-lactic acid) (pPLLA) membrane. Freshly excised PTSs bound well to the highly porous pPLLA membrane, possibly due to the presence of semihemispheric 20-30 mu m diameter openings on the upper surface. Global gene expression analysis demonstrated that periosteal sheets cultured on pPLLA membranes upregulated expression of many adhesion molecules. Osteogenic induction stimulated the production of proteoglycans by these cells and concomitantly enhanced their expansion and penetration into the deep pore regions of the membrane in parallel with the progression of in vitro mineralization. These findings suggest that our pPLLA membranes not only facilitate initial adhesion, primarily mediated by adsorbed proteins, but also enhance biological adhesion by inducing endogenous adhesion molecules in periosteal sheet cultures. Therefore, the efficacy of periosteal sheets in therapy should be greatly enhanced by using this new pPLLA membrane. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 98A: 100-113, 2011.