A monoclonal antibody against chicken thrombocytes reacts with the cells of thrombocyte lineage
A monoclonal antibody against chicken thrombocytes reacts with the cells of thrombocyte lineage
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DOI：
10.1292/jvms.66.243
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发表时间：
2004-03-01
影响因子：
1.2
通讯作者：
Matsuda, H
中科院分区：
文献类型：
--
作者：
Horiuchi, H;Tanaka, K;Matsuda, H
制备了一种新的抗鸡外周血血小板单克隆抗体HUKT。mAb HUKT似乎检测鸡血小板表面上的特异性标志物。流式细胞仪分析表明，该蛋白与正常胸腺细胞、法氏囊细胞、6种鸡细胞系细胞、鸡红细胞和人血小板均不发生反应。外周血白细胞（PBL）中HUKT+细胞为CD 45（低）、Bu-la（-）和CD 3（-）细胞。免疫印迹分析表明，HUKT识别的分子是一个单体，在非还原和还原条件下的表观分子量为150 kDa。组织分布研究表明，HUKT只对骨髓中的血小板系细胞和胚胎血细胞染色。HUKT单克隆抗体可用于鸡血小板谱系的个体发育研究和免疫学研究。
A new mouse monoclonal antibody (mAb), HUKT was raised against chicken peripheral blood thrombocytes. The mAb HUKT appeared to detect a specific marker on the surface of chicken thrombocytes. Flow cytometry (FCM) analysis revealed that it did not react with cells from the normal thymus, bursa of Fabricius, six kinds of chicken cell lines, chicken erythrocytes or human platelets. In addition, HUKT+ cells in peripheral blood leukocytes (PBL) were CD45(low), Bu-la(-) and CD3(-) cells. Immunoblotting analysis showed that the molecule recognized by HUKT is a monomer with an apparent molecular weight of 150 kDa under non-reducing and reducing conditions. Tissue distribution studies revealed that only cells of thrombocyte lineage in bone marrow and embryonic blood cells Were stained by HUKT. The HUKT mAb presented here may be useful for both ontogenetic studies of thrombocyte lineage and immunological studies in the chicken.