The Cortical Ischaemic Penumbra Associated with Occlusion of the Middle Cerebral Artery in the Cat: 2. Studies of Histopathology, Water Content, and in vitro Neurotransmitter Uptake
The Cortical Ischaemic Penumbra Associated with Occlusion of the Middle Cerebral Artery in the Cat: 2. Studies of Histopathology, Water Content, and in vitro Neurotransmitter Uptake
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与猫大脑中动脉闭塞相关的皮质缺血半暗带：2. 组织病理学、含水量和体外神经递质摄取的研究
DOI：
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发表时间：
1983
影响因子：
6.3
通讯作者：
J. A. Hardy
中科院分区：
文献类型：
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作者：
A. Strong;B. Tomlinson;G. Venables;G. Gibson;J. A. Hardy
在大脑中动脉闭塞的猫的边缘回中检查了缺血半暗带的性质，定义为在稳态软脑膜表面钾活性不增加超过13 mM的情况下脑电图振幅的抑制。研究了光和电子显微镜水平下的体外突触体神经递质摄取、含水量（比重）和组织病理学，并将结果与更深、临界缺血水平（小于 15 ml 100 g−1 min−1）获得的结果进行比较。边缘回 (NS) 中的 [3H]4-氨基丁酸摄取量为对照的 104%，严重缺血时为 61% 和 48% (p < 0.05)。结论是，体内突触体摄取受损是神经元同时广泛损伤的标志，而不是仅限于突触室的变化，尽管目前的发现不能排除体内可逆的、底物限制的摄取受损。仅在严重缺血时才观察到比重降低。在 6 个实验中的 5 个中，在光学显微镜下在边缘回的限制区域中观察到早期或经典的缺血性神经元细胞变化和反应性神经胶质细胞，无论是在微病灶中还是更分散地分散。超微结构变化更为频繁，但被认为只影响少数神经元。讨论了半暗带选择性电生理抑制的假设。
The nature of the ischaemic penumbra, as defined by suppression of electroencephalogram amplitude in the absence of increase in steady state pial surface potassium activity in excess of 13 mM, was examined in the marginal gyrus of cats subjected to middle cerebral artery occlusion. In vitro synaptosomal neurotransmitter uptake, water content (specific gravity), and histopathology at the light and electron microscopic level were studied and the results compared with those obtained at deeper, critical levels of ischaemia (less than 15 ml 100 g−1 min−1). [3H]4-Aminobutyric acid uptake was 104% of control in the marginal gyrus (NS), and 61 and 48% (p < 0.05) in critical ischaemia. It is concluded that impairment of in vivo synaptosomal uptake is a marker of simultaneous widespread damage to neurones, rather than of a change restricted to the synaptic compartment, although the present findings cannot exclude reversible, substrate-limited impairment of uptake in vivo. Reductions in specific gravity were seen only with critical ischaemia. In 5 of 6 experiments, early or classical ischaemic neuronal cell changes and reactive glia were seen on light microscopy in restricted areas in the marginal gyrus, either in microfoci or scattered more diffusely. Ultrastructural changes were more frequent but were considered to affect only a minority of neurones. Hypotheses for selective electrophysiological suppression in penumbra are discussed.